FLUTAX法显示纤毛虫微管胞器的改良  

An Improved FLUTAX Method for Visualizing the Microtubule Organelle of Ciliates

作　　者：何兰[1] 曾红[1] 沈洁[1] 牛延宁[1] 娄慧玲[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《动物学杂志》2006年第3期59-61,共3页Chinese Journal of Zoology

基　　金：国家自然科学基金资助项目(No.30470201)

摘　　要：在参考Arregui等报道的方法的基础上,对紫杉醇荧光染色(FLUTAX)显示纤毛虫细胞微管胞器的流程进行了改进,对其中一些试剂的配方作了调整,改进了多聚赖氨酸涂布玻片、皂苷孵育、多聚甲醛固定和Triton X-100渗透等步骤。A more effective improved method for visualizing the mierotubule organelle of eiliates with FLUTAX has been established, based on the procedure described by Arregui et al. Four main improved steps are coating slides with polylysine, incubating cells with saponin, fixing cells with polyformaldehyde and infiltrating cells with Triton X-100.

关 键 词：纤毛虫 微管胞器 FLUTAX方法 

分 类 号：Q954-33[生物学—动物学]