中药脂肝宁预防酒精性脂肪肝的实验研究  

EXPERIMENTAL STUDY ON THE MECHANISM OF ZHIGANNINGS PREVENTION OF ALCOHOLIC FATTY LIVER

作　　者：周俊英[1] 姚树坤[2] 张瑞星[2] 

机构地区：[1]河北医科大学第三医院感染科,石家庄050051 [2]河北医科大学第四医院消化科,石家庄050014

出　　处：《中国组织化学与细胞化学杂志》2006年第3期328-332,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(30070371)

摘　　要：目的探讨中药脂肝宁预防酒精性脂肪肝的作用机理。方法应用酒精灌胃方法建立大鼠酒精性脂肪肝模型,造模同时给予中药脂肝宁(ZGN)药物干预,以硫普罗宁(TPN)为对照,检测血清肝功能和肝匀浆甘油三酯(TG)、氧自由基(ORF)、抗超氧阴离子(ASOA)、超氧化物歧化酶(SOD)及丙二醛(MDA)含量,并对大鼠肝脏行病理学检查。结果酒精灌胃12周后大鼠形成酒精性脂肪肝,中药脂肝宁高浓度组血清门冬氨酸氨基转移酶(AST)和肝匀浆TG、ORF及MDA含量显著降低,而ASOA和SOD含量升高(P<0.05),肝脏脂肪变明显改善。结论中药脂肝宁通过抑制氧化应激、稳定肝细胞膜、抑制脂质过氧化来改善酒精所致肝细胞脂肪变性。Objective To study the mechanism of Zhiganning （ZGN） prevention in alcoholic fatty liver. Methods The rat models of alcoholic fatty liver were induced by intragastric alcohol. Meanwhile, the rats were treated with tiopronin （TPN） and ZGN. Serum hepatic function indexes and the level of triglyceride （TG）, oxygen free radical （ORF）, superoxide dismutase （SOD）, anti-superoxide anion （ASOA） and malondiadehyde （MDA） in the liver homogenate were determined respectively. Liver tissue was dyed with HE and sudan IV, and histopathological changes in the liver observed. Results Rat models of alcoholic fatty liver were induced successfully after intragastric alcohol for 12 weeks. In the ZGN groups, the level of AST in the serum, TG, ORF, and MDA in the liver homogenate of rats decreased significantly while the content of SOD and ASOA increased. The steatosis state of liver was improved. Concluaion ZGN improves alcoholic fatty liver by suppressing oxidative stress, stabilizing the hepatic cellular membrane and inhabiting lipid peroxidation.

关 键 词：脂肝宁 酒精性脂肪肝 实验研究 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]