日本血吸虫合抱相关未知功能基因SJCHGC00821的克隆及生物信息学分析  

Molecular cloning and bioinformatic analysis of a novel pairing-associated gene SJCHGC00821 of Schistosoma Japonicum

作　　者：戴橄[1] 汪世平[1] 余俊龙[1] 姜孝新[1] 曾少华[1] 徐绍锐[1] 李文凯[1] 

机构地区：[1]中南大学病原生物学系,湖南长沙410078

出　　处：《中国现代医学杂志》2006年第11期1665-1668,共4页China Journal of Modern Medicine

基　　金：国家"十五"重大科技专项课题(No.2002AA2Z3343);国家863血防重大专项(No.2004AA2Z3530);湖南省"十五"重点学科建设专项经费联合资助

摘　　要：目的克隆与日本血吸虫合抱相关但未知功能的新基因SJCHGC00821,构建其真核表达载体,并应用生物信息学初步探讨其功能。方法应用PCR技术从日本血吸虫成虫cDNA文库中扩增SJCHGC00821基因全长ORF,将其亚克隆到真核表达载体pcDNA3中,通过PCR、限制性酶切分析及测序进行鉴定。应用生物信息学初步分析其细胞定位、蛋白序列、结构域及功能。结果获得日本血吸虫合抱相关未知基因SJCHGC00821的克隆,序列分析结果提示该cDNA序列含有一个597bp的完整阅读框序列,编码198个氨基酸,其编码蛋白的理论分子量为22.76kDa,等电点为4.84。二级结构分析预测提示其具有一定抗原性。结论成功地克隆了日本血吸虫SJCHGC00821基因全长ORF,构建了其pcDNA3真核表达载体,为进一步研究其结构与功能创造了条件。[Objective] To clone Schistosoma Japonicum SJCHGC00821, a novel pairing-assoeiated gene, and to construct its recombinant eukary-otie expression vector so as to explore its function and roles in pairing. [Methods] Schistosoma Japonicum SJCHGC00821 full length ORF was amplified by PCR from cDNA library of Schistosoma Japonicum. Recombinant eukaryotie expression veetor（peDNA3-SJCHGC） was then constructed by subcloning technique and confirmed by restriction enzyme digestion analysis and sequencing. Bioinformatic methods were used to analyze its possible structure and function. [Results] SJCHGC00821, with 597bp base pairs and coding for 198 amino acids, was amplified by PCR from eDNA library of Sehistosoma Japonieum. The eukaryotic expression vector corresponding to SJCHGC00821 （pcDNA3- SJCHGC）was successfully constructed,which was confirmed by PCR and sequencing. The molecular weight is 22.76 kDa and the theoretical pI is 4.84. Secondary structure analysis showd that this protein had antigenicity. [Conclusions] Schistosoma Japonicum SJCHGC00821 gene has been successfully cloned, which will benefit further research on its function as well as its implications in pairing of Sehistosoma Japonicum.

关 键 词：日本血吸虫 合抱相关基因 基因克隆 生物信息学 

分 类 号：R383.24[医药卫生—医学寄生虫学]