三七DNA指纹图谱分析  

Analysis of DNA Fingerprint Atlas of Radix Notoginseng

作　　者：赵熙[1] 李艳萍[1] 李顺英[2] 陈黎明[1] 杨小洁[1] 岳淑敏[1] 

机构地区：[1]云南省中医中药研究所,云南昆明650223 [2]昆明医学院,云南昆明650031

出　　处：《云南中医中药杂志》2006年第3期47-48,共2页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

摘　　要：目的:对文山三七和广西三七进行DNA指纹图谱的鉴别研究,了解其间遗传变异的大小,并建立三七品种的检定方法。方法:采用RAPD技术。结果:从45个引物中筛选出23个扩增稳定且谱带清晰的引物,共得到211个遗传标记。DNA指纹图谱显示,样品间有遗传差异,具有不同的遗传特征,据此可进行鉴别。结论:RAPD技术可作为三七鉴别的参考方法。Objective: To differentiate DNA fingerprint atlas of Wenshan notoginseng and Guangxi notoginseng and understand the size of their genetic variation and establish a detection method for the notoginseng species. Methods: RAPD technology was used. Results: 23 primers of steady increase and clear spectrum were selected from 45 primers. 211 genetic marks were obtained. DNA fingerprint atlas showed that the samples had genetic difference and different genetic feature, on which the differentiation could be made. Conclusion: RAPD technology can be taken as reference to differentiate notoginseng.

关 键 词：云南三七 广西田七 RAPD DNA指纹图谱 

分 类 号：R282.5[医药卫生—中药学]