CD81和趋化因子CC受体5与丙型肝炎合并人类免疫缺陷病毒感染的相关性研究  

Correlation of receptor CD81 and CCR5 with HCV/HIV co-infection

作　　者：康辉[1] 范霞[1] 刁莹莹[1] 姜拥军[1] 代娣[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001

出　　处：《中华检验医学杂志》2006年第5期398-401,共4页Chinese Journal of Laboratory Medicine

基　　金：国家"十五"科技攻关课题(2004BA719A12);辽宁省教育厅高等学校科学研究项目(2004C049)

摘　　要：目的检测外周血CD4+及CD8+T淋巴细胞表面CD81、CCR5的表达,探讨与丙型肝炎病毒(HCV)单纯感染、人类免疫缺陷病毒(HIV)单纯感染和HCV/HIV合并感染的关系。方法采用流式细胞术,检测HCV感染组(n=21)、HIV感染组(n=14)、HCV/HIV感染组(n=28)及正常对照组(n=30)人外周血CD4+及CD8+T淋巴细胞表面CD81和CCR5的表达。结果与正常对照组相比,外周血CD4+T淋巴细胞表面CD81,在HCV感染组表达变化不明显,而在HIV感染组和HCV/HIV合并感染组中低表达;CD8+T淋巴细胞表面CD81,在HCV感染组、HIV感染组和HCV/HIV合并感染组中都呈高表达。外周血CD4+T和CD8+T淋巴细胞表面CCR5,在HIV感染组高表达,而在HCV感染组和HCV/HIV合并感染组中低表达。结论HCV/HIV合并感染中,影响外周血CD4+及CD8+T淋巴细胞表面CCR5表达的主要因素是HCV感染;而合并感染对CD4+及CD8+T淋巴细胞表面CD81的表达,其影响作用是不相同的。Objective To investigate the expression of CD81 and CCR5 on CD4 ＋/CD8 ＋ T lymphocytes in the peripheral blood of patients with HCV mono-infection, HIV mono-infection and HCV/ HIV co-infection as well as the correlation between them. Methods We measured the expression of CD81 and CCR5 on CD4＋/CD8 ＋ T lymphoeytes in peripheral blood samples from 21 patients with HCV mono- infection, 14 patients with HIV mono-infection, 28 patients with HCV/HIV co-infection and 30 healthy volunteers by FACS. Results The expression of CD81 on CD4 ＋ T lymphoeytes in HCV mono-infection was not different from healthy control, but decreased significantly in HIV mono-infection and HCV/HIV co- infection. The expression of CD81 on CD8 ＋ T lymphoeytes increased significantly in HCV mono-infection, HIV mono-infection and HCV/HIV co-infection. The expression of CCR5 on CD4 ＋ and CD8 ＋ T lymphocytes increased significantly in HIV mono-infection, but decreased significantly in both HCV mono- infection and HCV/HIV co-infection. Conclusion In HCV/HIV co-infection, HCV may have effect on the expression of CCR5 on CIM ＋ and CD8 ＋ T lymphocytes, while, HCV/HIV co-infection may have different ,effect on the expression of CD81 an CD4 ＋ and CD8 ＋ T lymphocytes from poripheral blood.

关 键 词：受体 CR5 肝炎 丙型 HIV 

分 类 号：R512.63[医药卫生—内科学] R512.91[医药卫生—临床医学]