血小板-胶原相互作用过程中GPⅠa/Ⅱa的功能  

Function of GPⅠa/Ⅱa in the platelet-collagen interactions

作　　者：张卓[1] 丛玉隆[1] 涂植光[2] 

机构地区：[1]解放军总医院检验科,北京100853 [2]重庆医科大学检验系

出　　处：《中华检验医学杂志》2006年第5期428-430,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨GPⅠa/Ⅱa在血小板-胶原黏附过程和胶原诱导血小板颗粒释放反应中的功能。方法采用流式细胞术,在有或无抗GPⅠa/Ⅱa抗体阻断条件下,(1)用anti-CD42b-PE标识血小板并检测血小板与FITC荧光标记胶原黏附过程中FITC荧光强度的变化;(2)用anti-CD61-PerCP标识血小板并用PE标记的抗CD62P抗体检测胶原诱导血小板颗粒释放反应中CD62P的变化。结果静息加6F1组比静息组下降9·0%,活化加6F1组比活化组降低33·6%。抗GPⅠa/Ⅱa抗体能抑制血小板与胶原的黏附,对活化态血小板的抑制作用更明显(P<0·01);抗GPⅠa/Ⅱa抗体不能抑制胶原诱导的血小板释放反应(P>0·05)。结论GPⅠa/Ⅱa在血小板与胶原黏附过程中有重要作用,阻断GPⅠa/Ⅱa能降低血小板对胶原的结合,但阻断GPⅠa/Ⅱa难以抑制由胶原诱导的血小板颗粒内容物的释放。Objective To investigate the function of GP Ⅰ a/Ⅱ a in the platelet-collage adhesion and the platelet granules release induced by collagen. Methods With the flow cytometry, in either presence or absence of the anti-GP Ⅰ a/Ⅱ a antibody ： （ 1 ） PE-labeled anti-CD2b antibody was used for selecting platelet and FITC fluorescence intension was detected during the course of platelet adhered to FITC- labeled collagen. （2） PerCP-labeled anti-CD61 antibody was used for selecting platelet and detecting CD62P with PE-labeled CD62P antibody during platelet granule release induced by collagen. Results Anti- GP Ⅰ a/Ⅱ a antibody inhibited the platelet-collagen adhesion, especially the activated platelet-collagen adhesion （P 〈 0.01 ）. Platelet granule release induced by collagen could not be inhibited by anti-GP Ⅰ a/Ⅱ a antibody （P 〉 0. 05 ）. Conclusion GP Ⅰ a/Ⅱ a plays an important role in platelet-collagen adhesion. Blocking GP Ⅰ a/Ⅱ a can decrease the platelet-collagen adhesion, but has no effects on platelet granule release reaction.

关 键 词：血小板 胶原 流式细胞术 

分 类 号：R33[医药卫生—人体生理学]