纤溶酶原激活物抑制物-1与动脉粥样硬化关系的研究  

Study on relationship of plasminogen activator inhibitor-1 and atherosclerosis in rabbits.

作　　者：秦玲[1] 张家颖[2] 吴广恒[2] 祝雁[3] 

机构地区：[1]吉林大学第一医院心内科,长春130021 [2]吉林大学基础医学院生物化学与分子生物学实验中心 [3]吉林大学前卫医院内科

出　　处：《中国综合临床》2006年第7期577-579,共3页Clinical Medicine of China

基　　金：吉林省科技厅资助课题(20050408)

摘　　要：目的通过对动脉粥样硬化(AS)兔血清和粥样硬化斑块中纤溶酶原激活物抑制物-1(PAI-1)表达的研究,探讨PAI-1在AS中的作用。方法16只雄性大耳白兔随机分为正常饮食组和高脂饮食组,每组8只,饲养16周。两组白兔均于0周、16周取耳缘静脉血,检测血清中PAI-1的水平;16周后处死,应用免疫组化方法检测PAI-1在主动脉粥样硬化斑块中的表达。结果正常饮食组和高脂饮食组0周血清中PAI-1水平差异无显著性(P>0.05);高脂饮食组16周后血清PAI-1水平较0周显著增加(P<0.01);免疫组化结果显示高脂饮食组主动脉壁PAI-1的表达明显高于对照组(P<0.01)。结论动脉粥样硬化的发生伴有血清和粥样斑块中PAI-1表达增加,PAI-1可能参与了AS的形成。Objective To study the expression of plasminogen activator inhibitor -1 （ PAI-1 ） of the blood serum and plaques in atherosclerotic rabbits in order to explore the effect of PAI-1 on atherosclerosis （ AS ）. Metbotis 16 male white rabbits were randomly divided into normal diet group and high cholesterol diet group （n = 8 for each）. The rabbits were fed for 16 weeks. Blood was collected from the marginal vein of the ear in 0 and 16 weeks re- spectively in two groups. Concentration of PAI-1 was measured; After 16 weeks,the rabbits were sacrificed to investigate the expression of PAI-1 in aortic atherosclerosis by immunohistochemistry analysis. Results There were not obvious differences of PAI-1 in the normal diet group and the high-cholesterol diet group at 0 week （P 〉 0.05 ）, obvious increment of PAI-1 in the high-cholestrol diet group after 16 weeks than 0 week （ P 〈 0.01 ） ; the higher expression of PAI-1 in cholesterol diet group was higher than that in control group （ P 〈 0.01 ）. Conclusion PAI-1 expression is increased in blood serum and plaque , which is correlated with atherosclerosis, suggesting that PAI maybe involved in the formation of AS.

关 键 词：动脉粥样硬化 纤溶酶原激活物抑制物-1 

分 类 号：R543.5[医药卫生—心血管疾病]