青蒿素与转铁蛋白相互作用的光谱分析  

Spectrometric study on the interaction between Artemisinin and Transferrin

作　　者：蔡怀鸿[1] 杨培慧[1] 蔡继业[1] 

机构地区：[1]暨南大学化学系,广东广州510632

出　　处：《化学研究与应用》2006年第6期649-652,共4页Chemical Research and Application

基　　金：广东省自然科学基金(021190);广州市科技计划项目基金(2003Z3-D2041)

摘　　要：应用分子荧光光谱与紫外-可见吸收光谱法研究了青蒿素与转铁蛋白分子间的相互作用,发现青蒿素对转铁蛋白有荧光猝灭作用,并对其作用机理做了探讨。依据F ster非辐射能量转移理论,测定了在30℃时,转铁蛋白-青蒿素间的结合常数(K=1.74×105L/mol)和结合位点数(n=0.696),结合距离(r=1.94nm);并采用同步荧光技术考察了青蒿素对转铁蛋白构象的影响。The interaction of Artemisinin（QHS） and Transferrin（Tf） was studied by fluorescence measurement and UV absorption spectrometry. At 30℃, the results showed that the combination constant K = 1.74 × 10^5 L/mol , the number of binding sites n = 0. 696. The binding distance（r = 1.94nm） between QHS and Tf was obtained based on FOster spectroscopy theory. The effect of QHS on the conformation of Tf was further analyzed by using synchronous fluorescence spectrometry.

关 键 词：青蒿素 转铁蛋白 分子间相互作用 光谱分析 

分 类 号：O621.61[理学—有机化学]