南五味子中五味子甲素的薄层扫描色谱定量分析  

Study on the TLC determination of deoxyschizandrin in Schisandra Sphenanthera Rehd et Wils

作　　者：沈宁[1] 敬晓丽[2] 陈晓春[1] 何晗[1] 梁冰[1] 

机构地区：[1]四川大学化工学院,四川成都610065 [2]四川省卫生管理干部学院,四川成都610041

出　　处：《化学研究与应用》2006年第6期741-743,共3页Chemical Research and Application

摘　　要：The application of TLC scan technique using digital camera combining with image-processing software to the determination of Deoxyschizandrin in Schisandra Sphenanthers Rehd et Wils was investigated. The content of Deoxyschizandrin was determined to be 0.58% by the image-processing method,with an error less than 5% in comparison with the result ot 0.61% gained by CAMAG TLC SCANNER method.The average recovery rate of the two methods were 108.3% and 100.3% with corresponding RSD 5.1%,2.1%(n=3),espectively.Meanwhile,the extraction method was improved.Based on these results,the TLC quantitative analysis method using digital image-processing technique was proposed, which was easy to apply widely with convenient operation,economical equipment and good stability.The application of TLC scan technique using digital camera combining with image- processing software to the determination of Deoxyschizandrin in Schisandra Spbenanthers Rehd et Wils was investigated. The content of Deoxyschizandrin was determined to be 0. 58% by the image - processing method, with an error less than 5% in comparison with the result ot 0. 61% gained by CAMAG TLC SCANNER method. The average recovery rate of the two methods were 108. 3% and 100. 3% with corresponding RSD 5.1% , 2. 1% （n = 3 ）, espectively. Meanwhile, the extraction method was improved. Based on these results, the TLC quantitative analysis method using digital image -processing technique was proposed, which was easy to apply widely with convenient operation, economical equipment and good stability.

关 键 词：五味子甲素 定量分析 薄层扫描色谱 

分 类 号：O657.7[理学—分析化学]