Ad-LMP2重组腺病毒疫苗在恒河猴体内免疫效果的研究  

Immune responses in rhesus induced by recombinant adenovirus Ad-LMP2

作　　者：王湛[1] 周玲[1] 吴小兵 卢觅佳[3] 宣尧仙[3] 左建民[1] 李峰[3] 王琦[1] 叶树清[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052 [2]本元正阳基因技术股份有限公司 [3]浙江省医学科学院国家(浙江)新药安全评价研究重点实验室

出　　处：《中华实验和临床病毒学杂志》2006年第2期63-65,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家"八六三"资助项目(2003AA216070);国家"九七三"资助项目(2004C8518806)

摘　　要：目的 观察带有EBV-LMP2的非复制型Ad-LMP2重组腺病毒疫苗免疫恒河猴诱导的针对EBV-LMP2的特异性细胞和体液免疫应答.方法 分别使用高剂量（4.5×10^11VP/kg）、中剂量（1.5×10^11VP/kg）、低剂量（0.5×10^11VP/kg）三个剂量的Ad-LMP2重组腺病毒,肌内注射免疫恒河猴,每5 d免疫一次,共免疫6次,第7周时使用ELISPOT方法检测猴外周血细胞毒性T细胞应答,同时应用免疫酶方法检测血清中抗LMP2抗体.结果 3个剂量免疫恒河猴均可以诱导出有效的细胞免疫应答及一定的抗体应答,免疫应答水平的高低与病毒剂量的高低有一定的关系,较高剂量产生的细胞及体液免疫应答水平比低剂量的要高.抗腺病毒中和抗体和抗LMP2抗体免疫2周后就可以检测到,其中抗LMP2抗体在免疫3～4周时滴度较高,7周时则与3～4周时接近或有所下降.结论 非复制型Ad-LMP2重组腺病毒疫苗可以有效的诱导恒河猴产生EBV-LMP2特异性细胞和体液免疫反应.Objective To observe the LMP2 specific cellular and humoral immune responses after immunization with recombinant adenovirus Ad-LMP2 in rhesus. Methods The rhesuses were immunized with Ad- LMP2 through intra muscular injection in three groups, high dosage （4.5 ×10^11 VP/kg）, medium dosage （1.5 ×10^11VP/kg） and low dosage （0.5 ×10^11 VP/kg） groups. They were totally immunized six times at intervals of 5 days. The specific cellular immune responses were tested during the 7^th week by ELISPOT after immunization. And the titers of anti-LMP2 antibody were tested by EIA throughout the period of immunization. Results LMP2 induced specific cellular and humoral immune responses in all three dosage group. The potency of immune responses was related with the dosage of immunization. Higher dosage elicited more potent immune response. Both the neutralizing antibody to adenovirus and anti-LMP2 antibody could be detected from 2 weeks after immunization. They would reach the peak during 3-4 weeks after immunization, then declined during the 7^th week after immunization. Conclusion The recombinant adenovirus LMP2 could induce specific cellular and humoral immune responses in rhesus after immunization.

关 键 词：腺病毒 重组 EPSTEIN-BARR病毒 潜伏膜抗原2 恒河猴 免疫应答 

分 类 号：R392[医药卫生—免疫学]