φχ174D、T4和f2噬菌体对碘伏消毒剂抵抗力的研究  

Comparative study on the resistance of Bacteriophage φχ174D, T4 and f2 to an iodophor in laboratory

作　　者：陈昭斌[1] 许欣[1] 张朝武[1] 朱敏[1] 王国庆[1] 孙巍[1] 

机构地区：[1]四川大学华西公共卫生学院医学检验教研室

出　　处：《中华实验和临床病毒学杂志》2006年第2期78-80,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：深圳市科技和信息局立项项目(深科信[2005]227号;序号244)

摘　　要：目的筛选出消毒实验中评价消毒因子对病毒灭活效果的指示噬菌体,比较研究了噬菌体φχ174D、T4和f2对碘伏消毒剂的抵抗力。方法消毒剂对噬菌体的灭活效果采用悬液定量灭活试验、中和剂鉴定试验,噬菌体的检测采用双层琼脂法。结果(1)有效碘浓度为500mg/L的碘伏溶液与噬菌体φχ174D作用40min,或有效碘浓度为750mg/L,作用10min,或有效碘浓度为1000mg/L,作用5min即可达到消毒水平[对噬菌体φχ174D的灭活对数值(LIV)或噬菌体φχ174D的对数减少值(LRV)(log10No-log10Nt)≥4·00log10]。(2)有效碘浓度为600mg/L的碘伏溶液与噬菌体T4作用40min,或有效碘浓度为700mg/L,作用5min即达到消毒水平。(3)有效碘浓度为50mg/L的碘伏溶液与噬菌体f2作用10min,或有效碘浓度为75mg/L,作用10min或有效碘浓度为100mg/L,作用5min达到消毒效果。结论上述三种噬菌体对碘伏的抵抗力由强到弱为:噬菌体φχ174D>噬菌体T4>噬菌体f2。Objective To screen for the most resistant bacteriophage as indictor in disinfection tests, the resistance of bacteriophage φχ174D, T4, and f2 to iodophor were obsvered in laboratory. Methods The virucidal activity of iedophor against bacteriophage φχ174D, T4, and f2 were assessed by suspension test. The neutralizer is selected and appraised by testing with neutralizer. Bacteriophage φχ174D, T4, and f2 were detected and enumerated by the double-agar-layer plaque technique. Results （ 1 ） With 500 mg/L of available iodine of iedophor solution, within a contact time of 40 min, or 750 mg/L, 10 min, or 1000 mg/L, 5 min, the reduction of bacteriophage φχ174D could achieve the ＂disinfection＂ level [log10 inactivation value （LIV） or log10 reduction value （LRV） of bacteriophage φχ174D （log10 No-log10 Nt） was ≥4.00 log10 ]. （2） With 600 mg/L of available iodine of iodophor solution, within a contact time of 40 min, or 700 mg/L, 5 min, the reductions of bacteriophage T4 could achieve the ＂disinfection＂ level. （3） With 50 mg/L of available iodine of iodophor solution, within a contact time of 10 min, or 75 mg/L, 10 min, the reductions of bacteriophage f2 could achieve the ＂disinfection＂ level. Conclusious The order of resistance of the above three bacteriophages to iodophor from greatest to smallest is as follows： bacteriophage φχ174D 〉 bacteriophage T4 〉 bacteriophage f2.

关 键 词：细菌噬菌体T4 细菌噬菌体φχ174 噬菌体F2 碘伏 抵抗力 

分 类 号：R187[医药卫生—流行病学]