金钗石斛试管苗生根研究  

Study on Radication of the Test-Tube Seedling of Dendrobium nobile

作　　者：黎建玲[1] 黄肇宇[1] 詹源庆[1] 蒋波[1] 

机构地区：[1]玉林师范学院化学与生物系,广西玉林537000

出　　处：《广西科学院学报》2006年第2期87-89,共3页Journal of Guangxi Academy of Sciences

基　　金：广西教育厅科研经费资助项目[2003(22号)]

摘　　要：在基本培养基MS上分别添加不同浓度和配比的α-奈乙酸（NAA）和6-苄氨基嘌呤（6-BA）、吲哚丁酸（IBA）和6-BA，不同浓度的多效唑（PP333）；在MS＋6-BA0．2mg·L^-1＋NAA1．0mg·L^-1＋活性炭0．1％培养基上分别添加10％的香蕉汁、马铃薯汁、椰子汁、番茄汁和胡萝卜汁5种天然成分对金钗石斛（Dendrobium nobile）试管苗进行生根研究。结果表明：NAA和IBA分别与6-BA配合使用均能抑制试管苗根的分化及生长，但单独使用对促进试管苗根的分化及生长有显著的效果，NAA的浓度为0．5～1．0mg·L^-1时，试管苗生根效果较佳，当IBA浓度为0．2mg·L^-1时，试管苗生根率达100％，且根数多而粗壮，NAA和IBA均可单独作为促进金钗石斛试管苗生根培养的理想的植物激素；PP3332～4mg·L^-1可促进再生植株根的形成，但抑制根的伸长生长，建议与其他激素配合使用；5种天然成分均对试管苗根的分化、长度和粗壮度有明显的促进作用，其中附加10％香蕉汁对试管苗的生根效果最好。We had researched the radication of the test-tube seedling of Dendrobiurn nobile through adding different concentration and proportion of combination of NAA and 6 BA,IBA and 6 BA, as well different concentration of PP333 into the basic culture medium of MS and through adding 10% banana juice,potato juice, coconut juice,tomato juice and carrot juice respectively into the culture medium of MS＋6-BA 0. 2 mg · L^-1＋NAA 1.0 mg · L^-1＋active carbon 0. 1 percent. Results. the mixture of 6 BA had mixed with NAA and IBA respectively could restrain the differentiation and growth of the test-tube seedling＇s root,but by using NAA and IBA singly, they had took remarkable effect on accelerating the differentiation and growth of the test-tube seedling＇s root. When the concentration of NAA had reached 0. 5 - 1.0 mg · L^-1 it was an advantage for test-tube seedling radication. When the concentration of IBA was 0. 2 mg· L^-1, the rate of radication of the test-tube seedling was 100 percent, and the amount of the roots was very much and the roots of the test-tube seedling were stronger. In addition, NAA and IBA both were fine hormone for accelerating the radication of the test-tube seedling. PP333 2- 4 mg · L^-1 promoted the development of regenerated plant root,but the root length growing was restrained, we proposed that it should associate with the other hormone ; Five kinds of natural composition could remarkahly accelerate the differentiation, the length and the strong of the test-tube seedling＇s root;adding in 10% banana juice had the best effect than others.

关 键 词：金钗石斛 试管苗 生根 

分 类 号：Q945.5[生物学—植物学]