HPLC/MS/MS法测定比格犬血浆中蒿甲醚及其代谢物双氢青蒿素的浓度  

HPLC/MS/MS determination of artemether and its metabolite dihydroartemisinin in Beagles'plasma

作　　者：刘萍[1] 侯禹男[1] 单成启[1] 唐利安[1] 王忠伟 程远国[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]通化同济医院,吉林134000

出　　处：《药物分析杂志》2006年第5期585-588,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立快速、灵敏液相色谱-串连质谱法测定血浆中蒿甲醚及其活性代谢产物双氢青蒿素浓度。方法:0.5mL 血浆样品经液液萃取后,以乙腈-0.1%醋酸(66:34)为流动相,采用 C_(18)预柱,通过电喷雾离子化三重四极杆串连质谱,以多重离子反应监测模式进行检测,m/z 321.1→m/z 275.1(蒿甲醚,ARM),m/z 284.3→m/z 267.1(双氢青蒿素,DHA),m/z 283,1→m/z 209.2(青蒿素,ART,内标)。结果:ARM 和 DHA 的线性范围为1～400ng·mL^(-1),日内、日间精密度均小于9.0%。结论:经方法学确证和稳定性评价,该方法成功应用于动物血浆中蒿甲醚和双氢青蒿素的定量测定和药代动力学研究。Objective： To establish an HPLC/MS/MS method for the determination of artemether （ARM）and its metabolite dihydroartemisinin（DHA） in Beagles＇ plasma. Method：ARM and DHA were extracted from plasma with liquid -liquid extraction. The mobile phase consisted of acetonitrile -0. 1% acetic acid （66： 34）. Electrospray ionization source was applied and operated in positive ion mode. Multiple reaction monitoring mode with the transition of m/z 321.1→m/z 275.1 ,m/z 284. 3→m/z 267. 1 and m/z 283. 1→m/z 209. 2 was used to quantify ARM ,DHA and artemisinin （ ART, internal standard） respectively. Results： The linear range of ARM and DHA was 1 - 400 ng ·mL^-1. The intra -day RSD and inter- day RSD was both less than 9. 0%. Conclusion：The established method is suitable for application in the determination of ARM and its metabolite DHA and investigation of ARM pharmacokinetics.

关 键 词：蒿甲醚(ARM) 双氢青蒿素(DHA) 青蒿素(ART) 液质联用 

分 类 号：R917[医药卫生—药物分析学]