HPLC法测定洋金花中东莨菪碱的含量  

HPLC determination of scopolamine hydrobromide in Flos Daturae

作　　者：柴金玲[1] 李瑛[1] 李伟[1] 谷学新[1] 

机构地区：[1]首都师范大学化学系,北京100037

出　　处：《药物分析杂志》2006年第5期634-636,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立 HPLC 测定洋金花中东莨菪碱的含量的方法。方法:采用 BDS HYPERSIL-C_(18)柱(5μm,3.0mm×250mm),流动相为:0.02mol·L^(-1)醋酸钠溶液(含0.02%三乙胺,用醋酸调 pH 至7.0)-甲醇(48:52),流速为0.5mL·min^(-1),检测波长为210nm,柱温:25℃。结果:东莨菪碱在0.6～2.0μg范围内具有良好的线性关系,r=0.9999,平均回收率为99.93%,RSD=1.0,2%(n=6)。结论:本法简便,准确,灵敏度高,重复性好,可为评价洋金花质量提供依据。Objective： To establish a method for determination of scopolamine hydrobromide in Flos Daturae by HPLC. Method ：The HPLC system consisted of BDS HYPERSIL -Clscolumn（5 μm,3.0 mm × 250 mm） ,0. 02 mol ·L^-1 sodium acetate （ contained 0. 02% triethyl amine, adjusted to pH = 7.0 with acetic acid ） - methanol （48： 52 ） mixture as a mobile phase,detection wavelength at 210 nm,0. 5 mL ·min^-1 of flow rate and column temperature at 25 ℃. Results： The linear range of scopolamine hydrobromide was 0. 6 - 2. 0 μg, r = 0. 9999, the recovery was 99. 93% ,RSD = 1.02% （n = 6 ）. Conclusion： The method is simple ,the result is accurate, higher sensitivity and the reproducibility is good. This method can be used for the quality study of Flos Daturae.

关 键 词：洋金花 氢溴酸东莨菪碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]