HPLC法测定香附二氧化碳超临界流体萃取物中α型β-香附酮与香附烯酮含量  

Determination of contents of β-cyperone and cyperotundone in Rhizoma Cyperi CO_2 supercritical fluid extracts by HPLC

作　　者：侯晓虹[1] 李岩[2] 唐星[1] 张悦杨[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学制药工程学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2006年第6期373-375,共3页Journal of Shenyang Pharmaceutical University

摘　　要：目的测定香附二氧化碳超临界流体萃取物中α型β-香附酮和香附烯酮的含量。方法采用HPLC法。色谱柱：Diamonsil C18柱（200mm×4．6mm，5μm）；流动相：甲醇-水（体积比为70：30）；流速：0．8mL·min^-1；检测波长：247nm；柱温：40℃。结果α型β-香附酮进样量在20．84-208．4ng内线性关系良好（r=0．9999），平均回收率为98．4％（RSD=1．83％，n=6）；香附烯酮进样量在5．220-52．20ng内线性关系良好（r=0．9999），平均回收率为99．0％（RSD=2．12％,n=6）。3批供试品中α型β-香附酮和香附烯酮含量的质量分数分别为11．96％（RSD=1．22％，n=3）、11．31％（RSD=1．24％，n=3）、11．30％（RSD=0．96％，n=3）和2．23％（RSD=1．62％．n=3）、2．27％（RSD=1．16％，n=3）、2．15％（RSD=1．42％，n=3）。结论HPLC法可同时测定香附二氧化碳超临界流体萃取物中α型β-香附酮和香附烯酮的含量；供试品中α型β-香附酮和香附烯酮与其他组分的色谱峰分离度良好。Objective To determine the contents of fl-cyperone and cyperotundone in Rhizoma Cyperi CO2 supercritical fluid extracts by reversed-phase HPLC method. Methods The sample was separated on a C18 column with the mobile phase of methanol in water solution （V： V = 70：30）. The flow rate was 0.8 mL.min^-1 and the detection wavelength was set at 247 nm. Results The calibration curve was linear in the range of 20.84- 208.4 ng for β-cyperone（ r = 0. 999 9） and 5.220- 52.20 ng for cyperotundone（ r=0. 999 9）. The average recoveries of fl-cyperone and cyperotundone were 98.4 % （RSD = 1.83 %, n = 6） and 99.0 % （RSD = 2.12 %, n = 6）, respectively. The contents of β-cyperone and cyperotundone in three batches of the Rhizoma Cyperi CO2 supercritical fluid extracts were 11.96% （RSD = 1.22%, n = 3）, 11.31% （RSD= 1.24%, n = 3）, 11.30% （RSD= 0.96%, n = 3）,2.23% （RSD= 1.62%, n = 3）,2.27% （RSD= 1.16 %, n = 3）, 2.15 % （RSD = 1.42 %, n = 3）, respectively. Conclusions The method is simple and accurate. It can be used as a method of quality control for products containing fl-cyperone and cyperotundone.

关 键 词：香附 二氧化碳超临界流体 萃取物 α型β-香附酮 香附烯酮 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]