心肌检测标志物——肌红蛋白分离纯化及亲和配体的研究  

The study on purification and affinity ligand of myoglobin—a new maker for detection of AMI

作　　者：马玉芹[1] 张淑华[2] 范丽颖[2] 董龙[3] 钱海刚[2] 丘瑜敏[2] 

机构地区：[1]长春理工大学材料与化工学院,吉林长春130022 [2]长春理工大学生命科学学院,吉林长春130022 [3]吉林大学环境科学与资源学院,吉林长春130021

出　　处：《东北师大学报（自然科学版）》2006年第2期114-117,共4页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20030450)

摘　　要：肌红蛋白主要存在于心肌和骨骼肌中,具有携带氧并将其分配到生物体各个部位的作用.研究表明,心血管病人发病早期,心脏中的肌红蛋白会大量释放入血,使其成为检测急性心肌梗死的良好标志物.采用HR100凝胶过滤层析和DEAE-52离子交换层析技术,从猪心中对肌红蛋白进行了分离与纯化,后经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定,得到了纯化的肌红蛋白,并利用Ph.D-7噬菌体展示肽库试剂盒对肌红蛋白特异性亲和配体进行了筛选,最终得到其特异性亲和配体序列,从而使其试剂盒的研制与应用成为可能.Myoglobin is composed of a heme prosthetic group and a polypeptide chain（globin） which have 152 residues. Myoglobin exists mainly in myocardium, cardiac muscle and the skeletal muscle. Myoglobin can store and assign the oxygen to all over the cell and tissue of the creature. It is proved recently that myoglobin can be used as a good biological marker for detection of acute myocardial infarction （AMI）. In this thesis, obtained myoglobin from bovine heart muscle using Sephacryl HR100 filtration chromatography and DEAE- Sephacel ion - exchange chromatography. After SDS - PAGE get the pure product. Myoglobin is a sensitive maker which can be used in diagnosis of the sudden death from myocardial ischemia. The specific sequence of affinity ligand was obtained with the Ph. D - 7 phage display peptide library kit. The results make it possible for clinical development and application of the kit.

关 键 词：肌红蛋白 HR100 纯化 肽库筛选 

分 类 号：Q513.4[生物学—生物化学]