日本血吸虫抗独特型抗体NP30抗体检测法在云南大山区应用效果的评价  

Potency of anti-idiotypic antibody NP30/antibody detection for schistosomiasis japonica in mountainous areas in Yunnan Province

作　　者：吴玉龙[1] 许宁[1] 仇镇宁[1] 陈凤 李远林 方文 杨杰 冯振卿[1] 管晓虹[1] 

机构地区：[1]南京医科大学卫生部抗体技术重点实验室 [2]云南省大理白族自治州血吸虫病防治研究所 [3]云南省大理白族自治州巍山县血吸虫病防治研究所

出　　处：《中国血吸虫病防治杂志》2006年第3期185-188,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家高技术研究发展计划(863计划)(2002AA214181)

摘　　要：目的评价日本血吸虫抗独特型抗体NP30抗体检测法在云南大山区血吸虫病流行现场的应用效果。方法对云南大山区血吸虫病流行区的506位居民进行粪检,同时用NP30抗体检测法和血吸虫抗体ELISA检测试剂盒(SEA-ELISA)分别对其血清进行检测,评价NP30抗体检测法的敏感性;同时检测非流行区的100份血清确定其特异性。结果NP30抗体检测法和SEA-ELISA法的粪检阳性符合率分别为87.80%(144/164)和84.76%(139/164);两者的特异性分别为96.00%(96/100)和94.00%(94/100),差异均无显著性(P均>0.05),但在粪孵阴性人群血清样本中,NP30抗体检测法和SEA-ELISA法的检出率分别为44.15%(151/342)和70.47%(241/342),差异有显著性(P<0.05)。结论NP30抗体检测法可用于云南大山区血吸虫病筛查。Objective To evaluate the potency of NP30 antibody detection for the diagnosis of schistosomiasis japonica in the mountainous endemic spots in Yunnan Province. Methods A total of 506 inhabitants in the mountainous schistosomiasis endemic regions of Yunnan Province were examined by using the stool hatching test for parasitological stool examination; the sera samples from 506 villagers were tested with NP30 antibody detection and SEA-ELISA for immune examination respectively, and the sensitivity of NP30 antibody detection was estimated . Their specificity was determined by testing 100 sera from nonepidemic scenes. Results The positive coincidence of stool hatching test with NP30 antibody detection and SEA-ELISA were 87.80% （144/164）and 84.76% （139/164） respectively, and their specificity as 96.00%（96/100）and 94.00%（94/100）, with no significant difference between them （P〉0. 05）. However, in the negative sera from stool hatching test, the detection rate was 44.15% （151/342） by NP30 antibody detection, and 70. 47%（241/ 342）by SEA-ELISA, the difference was significant（P〈0. 05）. Conclusion NP30 could be used in the mountainous area of Yunnan Province for the diagnosis of schistosomiasis japonica.

关 键 词：日本血吸虫病 抗独特型抗体 抗体检测 可溶性虫卵抗原-酶联免疫吸附试验 

分 类 号：R532.21[医药卫生—内科学]