胰高糖素样肽-1基因荧光真核表达载体的构建及鉴定  

Construction and identification of Eukaryotic Fluorescent Expression Plasmid of Glucagon - Like Peptide - 1 Gene

作　　者：张昌菊[1] 余云芳[1] 易凤华 

机构地区：[1]三峡大学医学院免疫学重点实验室,宜昌443002 [2]Div. of Cell and Molecular Biology ,Toronto General Research Institute, University of Toronto, Rm. 429, 67 College St, Toronto, Ontario, MSG 2M1, Canada

出　　处：《实用医学进修杂志》2006年第2期75-78,共4页Journal of Practical Training of Medicine

基　　金：湖北省教育厅国际合作项目（项目编号：053001）

摘　　要：目的：克隆大鼠胰高糖素样肽-1（GLP-1）的编码基因，构建荧光真核表达载体，为研究GLP-1与糖尿病的相关性奠定实验基础。方法：用RT-PCR方法从大鼠胰腺组织中扩增GLP-1的编码基因，克隆至PMD18-T载体并测序，结果正确后亚克隆至荧光真核表达载体pEYFP-N1中。结果：测序证实从胰腺中扩增出的GLP-1编码基因的序列及读框全部正确，重组质粒pEYFP-GLP-1经酶切后产生与理论预期长度相符的片段。结论：克隆了大鼠GLP-1的编码基因，成功构建了荧光真核表达载体pEYFP-GLP-1。Objective：To clone the coding sequence of rat GLP - 1 （ Glucagon - Like Peptide - 1 ） gene, construct its eukaryotic fluorescent expression plasmid, for the investigation of the relationship between GLP - 1 and diabetes. Methods： the GLP - 1 coding region was amplified from rat pancreas by RT - PCR, the PCR product was cloned into PMD18 -T vector, sequenced and then subcloned into vector pEYFP -N1. Results： Rat GLP - 1 was successfully amplified from pancreas, and expected fragment was digested from the recombined vector, pEYFP - GLP - 1. Conclusion ： Rat GLP - 1 coding rejoin, cloned from pancreas, was suc- cessfully constructed into pEYFP - N1.

关 键 词：胰高糖素样肽-1 pEYFP-N1 真核表达载体 

分 类 号：R318[医药卫生—生物医学工程] R587.1[医药卫生—基础医学]