TK基因突变试验检测叶绿素的抗诱变性  

DETECTION OF ANTIMUTAGENICITY OF CHLOROPHYLL BY TK GENE MUTATION TEST

作　　者：郭亚杰[1] 王亚男[1] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生教研室,成都610041

出　　处：《现代预防医学》2006年第6期861-862,867,共3页Modern Preventive Medicine

基　　金：国家自然科学基金(30471473);博士点基金(20020610036)资助项目

摘　　要：目的:用人的类淋巴母细胞TK6的TK基因突变试验检测叶绿素的抗诱变性。方法:不加S9活化的条件下,用叶绿素10、20、40、80μg/ml与阳性致突变物丝裂霉素C(MMC)0.5μg/ml同时处理TK6细胞3 h,采用微孔板法进行平板接种效率和突变频率的测定。结果:随受试物剂量的增加,叶绿素对MMC诱变性的拮抗作用增大,当剂量达到40μg/ml以上时总突变频率较MMC组有显著降低(P<0.05)。结论:叶绿素具有拮抗MMC诱导的tk基因突变的作用。Objective： To detect and evaluate the arttimutagenicity of chlorophyll by TK gene mutation test. Methods： The TK6 human lymphoid cells were exposed to chlorophyll at concentrations 10, 20, 40, 80μg/ml simultaneously, with 0. 5μg/ml MMC for 3 hours without S9. Determination of plating efficiency and mutant frequency were performed by using the microwell method. Results： A dose-dependent inhibiting effect of chlorophyll on MMC mutagenicity was found, and the mutant frequency depressed significantly compared with the MMC control when the concentration of chlorophyll arrived at 40μg/ml （P 〈 0.05 ）. Conclusiou： chlorophyll has obvious inhibiting effect on TK gene mutation induced by MMC.

关 键 词：TK6细胞 TK基因突变 叶绿素 

分 类 号：R994.4[医药卫生—毒理学]