抗人粒细胞单克隆抗体的制备鉴定和核素标记  

Preparation, characterization and nuclide labelling of antigranulocyte monoclonal antibody

作　　者：顾兆祥[1] 林祥通[1] 薛方平[1] 张锦明[1] 黄淳[1] 

机构地区：[1]上海医科大学附属华山医院

出　　处：《核技术》1996年第5期257-260,共4页Nuclear Techniques

基　　金：国家自然科学基金

摘　　要：用分离的人粒细胞免疫Ｂａｌｂ／ｃ小鼠，通过杂交瘤技术获得了１株分泌抗人粒细胞单克隆抗体的杂交瘤细胞（ＨＳＮ）。该抗体为ＩｇＧ１亚类．免疫组化法证实ＨＳＮＭｃＡｂ不仅选择地与人粒细胞结合，也与骨髓的粒细胞前体结合．但不与人淋巴细胞和红细胞以及其它动物的周围血细胞产生交叉反应。该抗体的亲和常数为５．７×１０８Ｌ／ｍｏｌ，每个粒细胞上的结合位点数为４．７×１０５个。９９^（９９）ｍＴｃ。The human granulocytes were isolated.Using hybridoma technique,a hybridoma cell line(HSN)producing monoclonal antibody against human granulocyte was obtained. The antibody belonged to IgG1 subclass.It was confirmed by immunohistochemical methods that HSN reacted selectively not only with human granulocytes,but also with their bone marrow precursors.Whereas human lymphocytes and red blood cells remained negative.No cross reaction was observed with peripheral blood cells in other animals.Its affinity constant was 5.7×108 L/mol,and the number of epitopes per granulocyte was 4.7×105.Monoclonal antibody displayed no loss of immunoreactivity after labelled with 99mTc.

关 键 词：人粒细胞 单克隆抗体 制备 核素标记 

分 类 号：R392－33[医药卫生—免疫学]