应用噬菌体肽库筛选NMDA受体抗原表位  

Selection of the minotope of NMDAR1 from phage displayed random peptide library

作　　者：李桂新[1] 孙长凯[2] 程桂芝[3] 罗建红[4] 李芳[3] 

机构地区：[1]大连医科大学附属第一医院检验科,116023 [2]大连医科大学脑疾病研究所 [3]大连医科大学免疫学教研室 [4]浙江大学医学院分子中心

出　　处：《河北医药》2006年第5期343-344,共2页Hebei Medical Journal

摘　　要：目的利用噬菌体展示随机肽库技术,进行NMDAR1抗原表位研究。方法以R1JHL单克隆抗体作为筛选分子,生物淘洗噬菌体展示随机12肽文库。经过4轮的筛淘,ELISA结合实验鉴定阳性克隆,提取阳性克隆噬菌体单链DNA进行序列分析。结果对阳性噬菌体进行递呈肽氨基酸序列分析,获得一阳性克隆序列HYNLSSDRKVRL,与R1JHL单克隆抗体识别序列相比,两者存在一致性序列DRK。结论DRK可能为NMDA受体抗原表位。Objective To select minotope of NMDAR1 by using phage displayed random peptide library. Methods R1JHLmcAb was used as target to screen the binding peptide from a 12-mer Ph. D. random peptide library. After four rounds of bipanning, the peptide sequence of positive clones was determined and analyzed by DNA sequencing and ELISA assay. Results A positive clone was found （HYNLSSDRKVRL）. There was a consentient sequence DRK in NMDAR1. Conclusion DRK in NMDAR1 might be the B cell epitope recognized by R1JHLMeAb.

关 键 词：噬菌体随机肽库 单克隆抗体 表位 NMDA受体 

分 类 号：R392.11[医药卫生—免疫学] R338[医药卫生—基础医学]