瓜类枯萎病菌粗毒素的致萎作用及其钝化研究  

Wilting Effect of Gourd Fusarium Wilt Pathogen and Its Inaltivation

作　　者：庄敬华[1] 杨长成[1] 高增贵[1] 毛竹[1] 唐树戈[1] 魏汉莲 董会[3] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]辽宁省中药研究所,沈阳110161 [3]山东农业大学,山东泰安271018

出　　处：《沈阳农业大学学报》2006年第2期177-181,共5页Journal of Shenyang Agricultural University

基　　金：辽宁省基金项目(20032087);辽宁省教育厅攻关项目(2004D218)

摘　　要：通过试验对瓜类枯萎病菌进行了产毒培养基的筛选,测定不同枯萎病菌菌株产毒能力,以及12种化合物对甜瓜枯萎病菌毒素的钝化作用。研究结果表明:改良的理查培养液最适于枯萎病菌的生长,其粗毒素对幼苗的致萎作用最强,并随着处理浓度的增加呈规律性变化;不同枯萎病菌菌株粗毒素对甜瓜幼苗的致萎作用差异显著;KMnO4、ZnSO4、VB6、VB1、CuSO4、CaSO4和MnSO4等均可不同程度地钝化甜瓜枯萎病菌毒素使其毒性降低。Four kinds of culture media for gourd Fusarium wilt pathogen were used for gourd Fusarium wilt pathogen to produce toxin and test the capability of different strains for producing toxin. Twelve compounds were also tested for inactivating the toxin of Fusarium wilt pathogen. The results was indicated that the improved Czapek culture medium was the most favorable for the pathogen growing, and its toxin had the strongest inhibition effect on seedling grow. With the increase of toxin concentration, the inhibition effect changed regularly. Different strains have a remarkable difference of inhibition melon seedling growth. The compounds, KMnO4 , ZnSO4 , VB6 , VB1 , CuSO4, CaSO4 and MnSO4 could inactivate the toxins of the pathogen in various degree.

关 键 词：瓜类枯萎病 毒素 萎蔫指数 细胞膜损伤率 钝化作用 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]