海墨止血片的质量标准研究  

Quality standard of Haimo Blood-Stanching Tablets

作　　者：姜云云[1] 范国荣[1] 叶光明[2] 

机构地区：[1]第二军医大学药学院上海市药物代谢产物研究重点实验室,上海200433 [2]中国人民解放军第102医院药械科,江苏常州213003

出　　处：《中成药》2006年第6期798-801,共4页Chinese Traditional Patent Medicine

摘　　要：目的：通过定性鉴别和含量测定制定海墨止血片的质量标准。方法：通过理化反应对黑色素进行定性鉴别；采用HPLC-AccQ-Tag法测定海墨止血片中甘氨酸、天门冬氨酸、谷氨酸和丙氨酸的含量，并采用原子吸收分光光度法测定微量铁元素的含量。结果：乌贼墨黑色素可溶于2mol／L氢氧化钠溶液，并在2mol／L盐酸溶液中有黑色飘浮状物析出；4种氨基酸和铁元素的加样回收率均在95％-105％之间，RSD均小于5％（n=6）。结论：该定性定量方法准确可靠，重复性好，可用来控制海墨止血片的质量。AIM： To establish the quality standard for Haimo Blood-Stanching Tablets （cuttle fish sepia） by means of quantitative and qualitative experiments. METHODS： Melanin was identified by physics and chemistry reaction. Gly, Asp, Glu and Ala in Haimo Blood-Stanching Tablets were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer. RESULTS： Melanin in sepia could be dissolved in 2 mol/L NaOH and there was something black separated out when in 2 mol/L HCl. The recoveries of four kinds of amino acids and iron were all in the range of 95%-105%. Each RSD was below 5% （n =6）. CONCLUSION： The quantitative and qualitative analysis methods are accurate and reproducible. This study provides a method for the quality control of Haimo Blood-Stanching Tablets.

关 键 词：海墨止血片 墨鱼汁氨基酸 铁元素 高效液相色谱法 原子吸收分光光度法 

分 类 号：R927.2[医药卫生—药学]