补肾益脑胶囊中补骨脂素和异补骨脂素的含量测定  

Determination of psoralen and isopsoralen in Bushen Yinao Capsules

作　　者：李学松[1] 范兴东 袁主邦 卓长城 李小林 

机构地区：[1]上海市脑血管病防治研究所,上海201318 [2]上海祥鹤制药厂,上海201318

出　　处：《中成药》2006年第6期810-812,共3页Chinese Traditional Patent Medicine

摘　　要：目的：建立补肾益脑胶囊（补骨脂，红参，茯苓等）中补骨脂素和异补骨脂素的含量测定方法。方法：采用HPLC法，用C18色谱柱，以甲醇-水（55：45）为流动相，检测波长247nm。结果：补骨脂素在0．038-0．309μg，异补骨脂素在0．036-0．295μg范围内线性关系良好，平均加样回收率：补骨脂素为99．50％，RSD为0．91％（n=5）；异补骨脂素为100．52％，RSD为1．70％（n=5）。结论：该法简便快速，准确实用，可用于控制补肾益脑胶囊的质量。AIM： To establish the method of determining psoralen and isopsoralen in Bushen Yinao Capsules （Fructus Psoraleae, Radix et Rhizoma Ginseng Rubra, Poria, etc. ）. METHODS： HPLC conditions were as follows： Chromasil C18 column, a mobile phase of CH3OH-H2O （55： 45）, and the wavelength at 247 nm. RESULTS： This method showed a good linear relationship in the ranges of 0.038 -0.309 μg （psoralen） and 0.036 -0.295 μg （isopsoralen）. The average recoveries were 99.50% for psoralen （ RSD was 0.91%, n = 5 ） and 100.52% for isopsoralen （RSD was 1.70%, n = 5 ）. CONCLUSION： The method is simple, swift, accurate and practical in quality control of Bushen Yinao Capsules.

关 键 词：补肾益脑胶囊 补骨脂素 异补骨脂素 HPLC 

分 类 号：R927.2[医药卫生—药学]