增强型绿色荧光蛋白表达对鼠C6细胞生物学特征影响的研究  

Effects of Expression of Enhanced Green Fluorencent Protein Gene on C6 Cells

作　　者：王有为[1] 宋玉光[2] 胡火珍[1] 林洪[1] 邢玉芝[1] 陈芬[1] 

机构地区：[1]四川大学生命科学学院细胞生物学教研室,成都610041 [2]四川省宜宾市第二人民医院,四川宜宾644000

出　　处：《四川大学学报（自然科学版）》2006年第3期655-660,共6页Journal of Sichuan University(Natural Science Edition)

摘　　要：脂质体包裹质粒pEGFP-N1转染C6细胞,经G418筛选,得到稳定表达绿色荧光的C6-EGFP细胞.细胞计数,MTT,流式细胞术分别测定C6细胞在转染绿色荧光蛋白后增殖性,细胞活力以及细胞周期等生物学特性.C6细胞在转染了绿色荧光蛋白基因后细胞增殖性降低,细胞活力降低,细胞周期也发生了一些改变.C6 cells were transfected with pEGFP-N1 by lipofectamine Plus. Stable EGFP-expressing cells were isolated with G418. Proliferation, activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry. As C6 cells transfected with pEGFP-N1, the proliferation and activity effects decreased and the cell cycle changed.

关 键 词：增强型绿色荧光蛋白 转染 C6细胞 MTT 流式细胞术 

分 类 号：Q25[生物学—细胞生物学]