Effect of Acupuncture at Acupoint Neiguan(PC6)on the Myocardial c-fos Gene Expression of Acute Myocardial Ischemia in Rats  

作　　者：谢芳 梁勋厂 吴红金 茹立强 朱忠春(译) 

机构地区：[1]Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China [2]Hospital of the Integrated Chinese and Western Medicine Affiliated to Beijing university of TCM, Beijing 1000130, P. R. China [3]不详

出　　处：《Journal of Acupuncture and Tuina Science》2006年第3期132-135,共4页针灸推拿医学（英文版）

基　　金：supported by research fund of science and technology of TCM,State Administration of TCM:2000-J-B-01

摘　　要：Objective：To investigate the mechanism of improvement effect of electro acupuncture at acupoint Neiguan （PC 6） on acute myocardial ischemia （AMI）. Methods： Ninety-six rats were randomly divided into sham-operation group, myocardial ischemia model group and myocardial ischemia model plus electro-acupuncture group, the rat model of AMI was established by occlusion of the anterior descending branch of the left coronary artery, then electro-acupuncture therapy was performed at bilateral acupoint Neiguan （PC 6）. The myocardial enzyme activities in plasma was detected with biochemical method, and the myocardial c-fos gent expression was detected with reverse transcription polymerase chain reaction（RT PCR） method. Results： The myocardial enzyme activities in plasma and the myocardial c-fos gene expression in myocardial ischemia model group were obviously higher than those in sham group（P〈 0.05）, after electro-acupuncture treatment, the myocardial enzyme activities in plasma and the myocardial c-fos gene expression were decreased, there was a significant difference （P〈 0.05）. Conclusion. The mechanism of improvement effect of electro acupuncture at acupoint Neiguan （PC 6） on AMI is related to down-regulation of the myocardial c-fos mRNA expression.目的:探讨电针"内关"改善急性心肌缺血的机制。方法:将96只大鼠随机分为假手术组、心肌缺血模型组、心肌缺血模型加电针组,采用结扎冠状动脉左前降支建立急性心肌缺血实验模型,造模成功后,电针双侧"内关"。用生化方法检测血清心肌酶活性,逆转录聚合酶链反应(RT-PCR)方法检测心肌中 c-fos 基因的表达。结果:心肌缺血模型组血清心肌酶活性和心肌 c-fos mRNA 表达显著高于假手术组(P<0.05),电针后降低,差异有显著性意义(P<0.05)。结论:电针"内关"改善急性心肌缺血的机制与下调心肌组织 c-fos mRNA 的表达有关。

关 键 词：ELECTRO-ACUPUNCTURE Point  PC 6 Myocardial lschemia Gene Expression 

分 类 号：R245.9[医药卫生—针灸推拿学]