荔枝转基因胚性愈伤组织原生质体分离条件的优化  

Optimization of the conditions for protoplast isolation from litchi transgenic embryogenic calli

作　　者：黄枝英[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2006年第3期266-271,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(39870544);霍英东高校青年教师基金资助项目(71026);福建省自然科学基金资助项目(C97030)

摘　　要：对荔枝品种下番枝转基因胚性愈伤组织原生质体分离条件进行了优化.结果表明:转基因胚性愈伤组织抗性细胞系Q811培养16-18 d后,转入含8 g.L-1纤维素酶Cellu lose R-10和4 g.L-1离析酶M acrease R-10的CPW+130 g.L-1甘露醇酶液中,在(25±2)℃、黑暗条件下,连续振荡(30-40 r.m in-1)10-11 h进行酶解,转基因原生质体产量达到18.2×106个.g-1,存活率达97.5%.In this experiment the transgenic embryogenic ealli with htp and gus were used as materials for optimizing the conditions for protoplast isolation in Litchi chimensis Soon. cv. Xiafanzhi. The results showed that litchi transgenic embryogenic calli after culture for 16-18 d were transferred into the CPW＋130g·L^-1 mannital enzymatic solution containting 8g·L^-1 Cellulose R-10, 4 g·L^-1 Macrease R-10 continuously shaking at 30-40r·min^-1 for 10 -11h at （25±2）℃ in darkness for innoculation; and the protoplast yield of 18.2×10s·g^-1 and viability of 97.5% were obtianed under the optimized procedures in the resistant cell line Q811.

关 键 词：荔枝 转基因胚性愈伤组织 原生质体 分离 优化 

分 类 号：S667.1[农业科学—果树学]