花叶病毒侵染的果蔗(Badila)的基因表达  

Gene expression in Badila infected by SCMV by means of differential display

作　　者：周以飞[1] 戴艳[1] 潘大仁[1] 陈观水[1] 高毅[1] 

机构地区：[1]福建农林大学作物科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2006年第3期288-291,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30370900);福建省自然科学基金资助项目(B0210017)

摘　　要：采用荧光标记差异显示技术(d ifferential d isp lay,简称DD-PCR)对甘蔗花叶病毒(Sugarcane m osaic virus,SCMV)侵染的果蔗(Bad ila)基因表达进行研究.用随机筛选出的23对引物组合扩增出9条差异条带,其中4条是有明显差异表达的cDNA条带,5条是表达强弱的差异条带.同时对一些影响荧光标记DD-PCR的因素进行了分析.Nine differentially expressed cDNA fragments were obtained with 23 primer combinations, the sizes of their fragments were from 250 to 350 bp. These fragments were divided into two types ： 4 cDNA fragments could differentially expressed in quality; 5 cDNA fragments could differentially expressed in intensity. Some factors affecting the technology were also analyzed.

关 键 词：果蔗 甘蔗花叶病毒 差异显示(DD) 基因表达 

分 类 号：S566.1[农业科学—作物学]