稻瘟菌T-DNA插入所致Pi9致病突变体的获得  

Magnaporthe grisea T-DNA insertional mutants virulent to Pi9

作　　者：林艳[1] 潘初沂[1] 李宏宇[1] 陈丽华[1] 鲁国东[1] 王宗华[1] 

机构地区：[1]福建农林大学教育部生物农药与化学生物学重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2006年第3期303-307,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30000111;30471132);福建省自然科学基金资助项目(B0520002)

摘　　要：利用自建稻瘟菌菌株FJ95054B T-DNA插入突变体库,筛选获得2个对水稻品种P i9致病的突变体P i9-2-8T940017401和P i9-1-2 T940032801.以T-DNA侧翼序列为探针筛选该菌株的BAC文库,得到阳性BAC克隆.Two mutants virulent to Pi9, Pi9-2-8 T940017401 and Pi9-1-2 T940032801, were obtained from the T-DNA insertional library of Magnaporthe grisea field strain FJ95054B by screening the rice variety with resistance gene Pi9. The positive clone in the BAC library was identified by using T-DNA flaking sequences as probes.

关 键 词：稻瘟病菌 T-DNA插入突变 Pi9致病突变体 阳性BAC克隆 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]