甘草Ri质粒转化及不同理化因子对甘草毛状根生长的影响  

Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots

作　　者：杨世海[1] 刘晓峰[2] 沈昕[3] 郑俊华[4] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林亚泰(集团)股份有限公司,吉林长春130031 [3]北京林业大学生物科学与技术学院,北京100083 [4]北京大学药学院,北京100083

出　　处：《中国中药杂志》2006年第11期875-878,共4页China Journal of Chinese Materia Medica

摘　　要：目的:研究甘草毛状根的诱导及外界因子对其生长的影响。方法:用发根农杆菌Agrobacterium rhizo-genesLBA9402和R1601感染甘草Glycyrrhiza uralensis外植体。结果:2种发根农杆菌均能诱导甘草产生毛状根,LBA9402比R1601表现出较强的对甘草的感染能力,下胚轴的转化率高于子叶。在WP培养基上毛状根生长最快。光照对毛状根生长有抑制作用。毛状根中5种黄酮类化合物的总量比愈伤组织中高1.5倍,其中甘草查耳酮含量是愈伤组织中的15.5倍。结论:本实验所建立的甘草毛状根培养系统,为研究甘草毛状根大量培养生产黄酮类化合物奠定了基础。Objective： To study the inducfion of hairy roots Glycyrrhiza uralensis and effect of some physicalas well as chemical factors on the growth of hairy roots. Method： Hypoctyls and cotyledons of G. uralensis were infected with Agrobacterium rhizogenes LBA9402 and A. rhizogenes R1601, respectively. Result： Hairy roots were induced by two strains of A. rhizogenes, and LBA9402 showed a stronger infective ability. The frequency of transformation of hypocotyls was higher than that of cotyledons. Among the different media tested, WP medium yielded the fastest growth rate of hairy roots. Light inhibited the growth of hairy roots. The total amout of 5 tlavonoids in hairy roots was 1.5 times as much as that in the callus, among them licochalcone content was 15.5 limes as that of callus. Conclusion： The vitro culture system of the hairy roots established laid a foundation for mass production of flavonoicls by hairy root culture.

关 键 词：甘草 发根农杆菌 毛状根 黄酮类化合物 

分 类 号：S567.71[农业科学—中草药栽培]