染料木黄酮对人卵巢癌裸鼠移植瘤影响的实验研究  

Regulative effect of genistein on xenografted tumor of ovarian carcinoma cell on nude mice

作　　者：王心[1] 辛晓燕[1] 黄艳红[1] 

机构地区：[1]第四军医大学西京医院,陕西西安710032

出　　处：《中国中药杂志》2006年第11期901-904,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30472226)

摘　　要：目的:探讨植物雌激素染料木黄酮对人卵巢癌HO-8910PM细胞裸鼠移植瘤的影响。方法:体外培养人卵巢癌HO-8910PM细胞,裸鼠皮下接种HO-8910PM细胞建立裸鼠移植瘤动物模型,随机分为对照组和3个染料木黄酮治疗组,治疗组分别给予5,25,50 mg.kg-1染料木黄酮。治疗4周后,应用流式细胞术(FCM)检测各组裸鼠移植瘤细胞周期时相和凋亡率,免疫组化技术检测凋亡基因bcl-2,Fas,FasL蛋白的表达情况,并通过电镜进行形态学观察。结果:50 mg.kg-1染料木黄酮治疗组裸鼠移植瘤明显小于对照组,肿瘤细胞G0-Gl期比例升高明显,S期细胞比例显著降低(P<0.01),移植瘤细胞凋亡率为(15.14±2.27)%,明显高于对照组(3.12±1.12)%(P<0.01);移植瘤细胞bc1-2蛋白表达水平明显下降,而Fas蛋白表达水平升高,与对照组比较,均有显著性差异(P<0.05)。电镜观察50 mg.kg-1染料木黄酮治疗组可见凋亡细胞明显增多。结论:染料木黄酮通过调节移植瘤细胞周期分布和凋亡基因,抑制卵巢癌细胞增殖,诱导细胞凋亡。Objective： To investigate the regulative effect of genistein on the apoptosis of the xenografted tumors of human ovarian carcinoma HO-8910PM cell on the nude mice. Method： Human ovarian carcinoma HO-8910PM were cultured in vitro. The models of xenografted tuner were established by the transplantation of human ovarian carcinoma HO-8910PM on nude mice. The nude mice were randomly divided into four groups of three treatment groups （in which genistein were administered ip at 5, 25 and 50 mg·kg^-1·d^-1 respectively, for 4 weeks） and one control group, the cell cycle and apoptosis of the xenografted tumors were measured by flow cytometry, the expression of bcl-2,Fas and FasL gene of xenografted ttanors were determined by the immunohistochemistry and the morphology of ttanor cells was observed by electron microscope. Result： The ttanor weights of 50 mg·kg^-1 genistein group were more lighter（ P 〈 0.05） compared to those of control group. The tumor cells in G0-G1 phase were increased, at saml time the cells in S-phase were decreased （ P 〈 0.01） after the treatment of 50 mg·kg^-1 genisrein. In addition the apoptosis rate of the cell treated with 50 mg·kg^-1 genistein group was （15.14 ± 2.27）%, which was significantly higher than that in the control group（3.12 ± 1.12）%（ P 〈0.01）. The expression of bcl-2 of the xenografted tumors was decreased and the expression of Fas was increased in 50 mg·kg^-1 genistein group, both showing a significant difference from those in control group （ P 〈 0.05）. The apoptosis of the ttanor cells were found more under electron microscopy in 50 mg·kg^-1 genistein group than those in control group. Conclusion： Genistein could significantly inhibite the proliferation and induce the apoptosis of the HO-8910PM cell of xenografted tumors by regulating the cell cycle and apoptoic gene in the nude mice.

关 键 词：染料木黄酮 卵巢癌 裸鼠 凋亡 

分 类 号：R285.5[医药卫生—中药学]