胞内抗体及其研究进展  

Intrabody and Its Latest Advances

作　　者：洪美亚[1] 曾冬云[1] 龚兴国[1] 

机构地区：[1]浙江大学生物大分子与酶工程研究所,杭州310027

出　　处：《细胞生物学杂志》2006年第3期425-428,共4页Chinese Journal of Cell Biology

摘　　要：胞内抗体是指在细胞内表达并被定位于亚细胞区室的一类新的工程抗体。目前胞内抗体的研究主要集中于ScFv,ScFv基因结构简单,易导入细胞内表达且便于体外重组操作。胞内抗体作为一种新的基因治疗工具,在肿瘤基因治疗、人艾滋病基因治疗的实验研究及潜在的临床治疗方面展示了广泛的应用前景。同时,胞内抗体可以用作分析靶蛋白功能的研究工具,是对传统的“基因剔除”转基因动物的一种有效补充。现从胞内抗体的设计及载体选择、肿瘤基因治疗、人艾滋病基因治疗等方面对胞内抗体的研究进展进行综述。As we all know, intrabody is a new engineering antibody which is expressed in cell and oriented in subcellular area. Due to ScFv gene＇s simple structure, it is amiable to expressed in cell and convenient for in vitro recombination operation. Accordingly current research on intrabody concentrate on ScFv. As a new gene therapy tool, intrabody brings forth extensive application foreground in the aspects of tumor gene therapy and human HIV gene therapy＇s experimental study and potential clinical therapy. At the same time, intrabody is also served as a study tool for analyse the function of target protein, and it＇s an effective complementarity to the traditional ＂gene knock- out＂ genetically modified animal. This article summarizes intrabody＇s latest research advances from its design and the selection of vector, tumor gene therapy and human HIV gene therapy.

关 键 词：胞内抗体 基因治疗 HIV-1 SCFV 

分 类 号：Q789[生物学—分子生物学]