葡萄糖浓度波动对原代培养的大鼠血管细胞和肾细胞的影响  

The Effect of Glucose Fluctuation on Primary Cultured Vascular Cells and Kidney Cells of Rat

作　　者：叶希韵[1] 金百胜[1] 申杰[1] 王耀发[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《细胞生物学杂志》2006年第3期491-494,共4页Chinese Journal of Cell Biology

摘　　要：探讨葡萄糖浓度波动对体外培养的原代大鼠血管细胞和肾细胞的影响。取SD大鼠的主动脉和肾脏进行血管细胞和肾细胞的体外原代培养,每种细胞均分为6组:正常对照组、持续高糖组、持续低糖组、波动组I、波动组II、波动组III。实验24h后,测定细胞乳酸脱氢酶(LDH)的泄漏率,细胞液中的β-N-乙酰氨基葡萄糖苷酶(NAG)的活力,细胞内还原型谷胱甘肽(GSH)和超氧化物歧化酶(SOD)的活力。结果表明葡萄糖浓度波动各组均能对大鼠血管细胞和肾细胞造成损伤,使细胞外液LDH、NAG的泄漏量明显增加,细胞内GSH、SOD活力明显减少,与持续高糖组和持续低糖组比较差异显著(P<0.001)。且葡萄糖浓度波动对肾细胞的损伤比血管细胞更为明显。说明葡萄糖浓度波动能够导致大鼠血管细胞和肾细胞的损伤,并且其损伤远远大于持续高糖或持续低糖的单独作用效果,损伤的结果与低糖作用细胞的时间呈正相关,在相同的损伤条件下肾细胞比血管细胞对葡萄糖浓度波动更为敏感,损伤更为严重。To explore the effect of glucose fluctuation on vascular cells and kidney cells. Use SD rat artery and kidney in primary culture vascular cells and kidney cells in vitro. Each kind of cells was divided into 6 groups as follows： control group, continuous high glucose group, continuous low glucose group, fluctuation group Ⅰ（each cycle includes low glucose 2 hours, high glucose 4 hours）, fluctuation group Ⅱ （low glucose 6 hours, high glucose 18 hours） and fluctuation group Ⅲ（low glucose 12 hours, high glucose 12 hours）. After 24 hours cultivation, we detected the leakage of LDH, NAG activity in each culture medium and activity of GSH and SOD in cells. The vascular cells and kidney cells were harmed in all fluctuation groups. The leakage of LDH and NAG increased evidently while the enzyme activity of GSH and SOD decreased obviously, compared with continuous high glucose group and continuous low glucose group （P〈0.001）. During the experiment, we found that the kidney cells are more sensitive to the damage of glucose fluctuation. Glucose fluctuation can do harm to rat vascular cells and rat kidney cells, and it also has relations with the time of low glucose cultivation. Under the same condition, kidney cells are more sensitive to the damages than vascular cells.

关 键 词：葡萄糖浓度波动 血管细胞 肾细胞 细胞培养 

分 类 号：Q813.11[生物学—生物工程]