新疆濒危植物盐桦试管苗生根培养的研究  

Rooting Cultivation of Rare Endangered Plant Betula halophila in Vitro

作　　者：梅新娣[1] 马纪[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院分子生物学重点实验室,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《新疆农业科学》2006年第3期218-223,F0003,共7页Xinjiang Agricultural Sciences

基　　金：国家科技攻关西部科技行动项目(2001BA901A32);国家"863"项目(2004AA227110-2)

摘　　要：采用L27(313)正交试验方法探讨了培养基种类A、植物生长调节剂及其不同浓度配比B、蔗糖量C、培养的光照条件D四因素对诱导盐桦生根(平均根长、生根系数、生根率)的影响。结果表明,生长素B、生长素×培养基(A×B)对生根系数有极显著的影响,培养基A、生长素B、生长素×培养基(A×B)仅对平均根长有极显著的影响,四个因子对生根率均无显著的影响。研究获得诱导盐桦平均根长的优化培养基为A2B1C3D1(即S27处理)MS+NAA 0.2 mg/L+蔗糖10 g/L+琼脂7%,诱导盐桦生根系数优化培养基为A3B2C3D2(与处理S21的A3B2C3D3相似)1/2MS+IBA 0.5 mg/L+蔗糖30 g/L+琼脂7%+暗光处理3 d,诱导盐桦生根率的优化培养基为A2B3C1D1(即S10处理)MS+IAA 0.2 mg/L+蔗糖30 g/L+琼脂7%。最佳生根培养基为:1/2MS+IBA 0.5mg/L+蔗糖30 g/L+琼脂7%+暗光处理3 d。其生根系数高达5.308,生根率高达96.30%以上。The four factors, such as A type of culture medium, B different concentrations of plant regulators, C content of sucrose , and D illumination condition, were tested for their effects on rooting of seedling of rare endangered plant Betula halophila in vitro in rooting coefficient, rooting rates and the average rooting length by orthogonal experiment method of L27 （3^13 ）. The results showed B and B × A had significant effect on rooting coefficient and the average rooting length, A has significant effect on the average roofing length. The rooting coefficient and the average rooting length had no significant difference as D and C were changed. The rooting rate had no significant difference as the four factors were changed. Based on these results, the optimization medium are as follows： A2B1C3D1（S27：MS ＋ NAA 0.2 mg/L ＋ sucrose 10 g/L） is the best medium for the average rooting length, A3B2C3D2（ similar to S21 - A3B2C3D3 ） （1/2MS ＋ IBA 0.5 mg/L＋ sucrose 30 g/L＋ three or seven days in dark ） for rooting coefficient, and A2B3C1D1（S10：MS＋ IAA 0.2 mg/L＋ sucrose 30 g/L） for rooting rates.

关 键 词：濒危植物 盐桦 正交试验 试管苗生根 

分 类 号：S792.15[农业科学—林木遗传育种] S722.37[农业科学—林学]