特发性脊柱侧凸双侧椎旁肌褪黑素受体含量的差异性比较研究  

Comparison of melatonin receptor mRNA expression in bilateral paravertebral muscles in adolescent idiopathic scoliosis

作　　者：邱勇[1] 吴亮[1] 王斌[1] 俞扬[1] 朱泽章[1] 钱邦平[1] 马薇薇[1] 

机构地区：[1]南京大学医学院附属鼓楼医院脊柱外科,210008

出　　处：《中华外科杂志》2006年第12期826-829,共4页Chinese Journal of Surgery

摘　　要：目的研究特发性脊柱侧凸椎旁肌中褪黑素受体含量的变化,并探讨其与特发性脊柱侧凸病因学的关系。方法本实验共分3组:特发性脊柱侧凸组:20例,平均Cobb角56°±16°,顶椎位于T6-11。其中Cobb角>50°10例,Cobb角≤50°10例。先天性脊柱侧凸组:12例,平均Cobb角59°±33°,顶椎位于T7-12。对照组:取10例非脊柱侧凸病例作为对照。采用RT-PCR方法检测所有病例两侧椎旁肌中褪黑素受体两种亚型MT1、MT2mRNA的表达量。结果特发性脊柱侧凸和先天性脊柱侧凸组顶椎区凹侧椎旁肌MT2mRNA的表达量明显小于凸侧(P<0·05),MT1mRNA的表达量两侧无显著差异(P>0·05)。特发性脊柱侧凸组中Cobb角>50°的病例顶椎区凹凸侧椎旁肌MT2mRNA表达量的比值与Cobb角≤50°的病例无显著差异(P>0·05)。对照组两侧椎旁肌MT1、MT2mRNA的表达量无显著差异(P>0·05)。结论特发性脊柱侧凸患者两侧椎旁肌MT2mRNA的表达量存在差异,这种差异可能为继发性改变,在特发性脊柱侧凸的发病中不起主要作用。Objective To study the change of melatonin receptor mRNA expression in paravertebral muscles in adolescent idiopathic scoliosis （AIS）, and analyze its relationship with the etiology of AIS. Methods Twenty cases with average age of（ 15.1±2. 2） years and average Cobb angle of 56°± 16°, including 10 cases with Cobb angle 〉 50° and 10 cases with Cobb angle ≤ 50°, were in AIS group. The apical vertebrae were from T6-11. Twelve cases with average age of （11.6±3.2）years and average Cobb angle of 59°-33°were in congenital scoliosis （CS） group. The apical vertebrae were from T7-12. Ten cases without scoliosis were in control group. The mRNA expression of melatonin receptor subtype MT1 and MT2 were detected by RT-PCR method. Results The MT2 mRNA expression of paravertebral muscles on concave side was higher than that on convex side in AIS and CS groups （P 〈 0. 05 ）, but the MT1 mRNA expression showed no significant clifference（P 〉0. 05）. In AIS group, the ratio of MT2 mRNA expression on concave side compared with convex side in cases with Cobb angle 〉50° and cases with Cobb angle≤50° showed no significant difference（ P 〉 0. 05 ）. The MT1 and MT2 mRNA expression showed no significant difference in control group（ P 〉 0.05 ）. Conclusions The melatonin receptor expression in bilateral paravertebral muscles in AIS is asymmetric, which may be a secondary change and has no important role in the pathogenesis of AIS.

关 键 词：脊柱侧凸 受体 褪黑素 椎旁肌 

分 类 号：R682[医药卫生—骨科学]