新型角膜支架材料的制备及生物相容性研究  

Preparation and Biocompatibility of a Novel Cornea Scaffold Material

作　　者：焦延鹏[1] 李立华[1] 丁勇 周长忍[1] 

机构地区：[1]暨南大学材料科学与工程系,广州510632 [2]暨南大学华侨医院,广州510632

出　　处：《生物医学工程学杂志》2006年第3期556-559,共4页Journal of Biomedical Engineering

基　　金：国家"863"资助项目(2001AA625050);"973"资助项目(G199054306);国家自然科学基金资助项目(30170272);广东省科技项目重大专项资助项目(A302020104)

摘　　要：首先合成了一种新型可降解的三乙烯基交联剂,并以偶氮二异丁氰(A IBN)为引发剂与乙烯基吡咯烷酮(NVP)通过自由基聚合制备了一种新型的交联NVP角膜支架材料。对材料的吸水率、接触角和降解性能进行了测定,通过体内埋植实验和细胞培养对材料进行了生物相容性评价。结果表明材料的吸水率达到104%,接触角为41,°降解速率较为恒定;体内埋植实验研究表明,3个月后,材料已大部分降解,材料内出现胶原和角膜基质细胞,周围组织无明显的炎症反应;细胞培养实验表明,角膜上皮细胞在材料上可以较好的生长,没有明显的细胞毒性,具有良好的生物相容性,这种合成的新型交联NVP角膜支架材料将在角膜组织工程中具有潜在的应用价值。A novel cornea tissue scaffold material was prepared with N-vinly pyrrolidome （NVP） and a biodegradable crosslinking agent by radical polymerization, using azoisobutyronitrile （AIBN） as initiator. Water absorption test and contact angle measure were conducted, and the degradation process of material was investigated. The biocompatibility evaluation was carried out by implantation of material in the rabbits, and by cell culture. The water absorption was over 104%, the contact angle was lower than 41°, and the degradation speed in vitro kept steady. The results of implantation in the rabbits showed that the material was almost degraded 3 months later and lots of collagen and cornea stroma cells appeared in it,but there was no inflammation around it. The result of epithelial cells culture showed that the cells conglutinated on the material, but no remarkable cytotoxicity was noted.

关 键 词：角膜 支架材料 制备 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]