甲壳素短纤维增强聚己内酯复合材料的制备及生物学评价  

Preparation and Biological Evaluation of Chitin Short Fiber Reinforced Polycaprolactone Composite

作　　者：段亮[1] 徐志飞[1] 孙康[2] 赵学维[1] 方嘉[2] 秦雄[1] 龚志云[1] 

机构地区：[1]第二军医大学附属长征医院胸心外科,上海200003 [2]上海交通大学复合材料研究所,上海200030

出　　处：《生物医学工程学杂志》2006年第3期565-568,共4页Journal of Biomedical Engineering

基　　金：上海市科学技术发展基金资助项目(024419076)

摘　　要：用共混法制备甲壳素短纤维增强聚己内酯复合材料,并对纯聚己内酯和甲壳素短纤维增强聚己内酯复合材料进行体外细胞毒性和生物相容性评价,为临床应用提供有价值的实验依据。对该两种材料进行体外细胞毒性试验、急性全身毒性试验、溶血试验、热源试验和过敏试验。结果显示受试材料最终细胞毒性级为0级,对细胞生长和增殖无明显抑制作用,材料中不存在潜在致敏性物质,浸提液无溶血反应和急性全身毒性反应,无热源反应,表明该复合材料具有良好的细胞和组织相容性,其作为胸壁缺损修补材料应用于临床具有可行性和安全性。Chitin short fiber reinforced polycaprolactone composite was prepared by melting blending method. The cytotoxicity and biocompatibility of pure polycaprolactone and of chitin short fiber reinforced polycaprolactone composite were investigated in order to provide useful scientific basis for clinical application. The biocompatibility of pure polycaprolactone and that of chitin short fiber reinforced polycaprolactone composite were evaluated by a series of tests, including cytotoxicity test in vitro, acute systemic toxicity test, hemolysis test, pyrogen test and sensitivity test. The results showed that the cytotoxicity scores of the two materials were grade 0 and the growth and proliferation of the cultured cells were not significantly inhibited by the two materials. There were no potential allergic materials in the composites and the maceration extract showed no hemolytic reaction, no acute systemic toxicity and no pyrogen reaction. We conclude that the composites have fine biocompatibility and are safe for clinical use in the reconstruction treatment for chest wall defect.

关 键 词：甲壳察纤维 增强 聚己内酯 细胞毒性 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]