胚体培养对小鼠ES细胞定向神经分化的影响研究  

The Effects of Embryonic Bodies on Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells in Vitro

作　　者：章淑芳[1] 章恩明[1] 郑筱祥[1] 

机构地区：[1]浙江大学生仪学院生物医学工程系,杭州310027

出　　处：《生物医学工程学杂志》2006年第3期620-624,共5页Journal of Biomedical Engineering

摘　　要：为了研究胚胎干细胞（ES细胞）的定向诱导分化过程中胚体的形成对其后分化的影响，通过悬滴培养、悬浮培养及两者结合的方法得到2～4d的胚体（EBs），利用全反视黄酸（RA）对其处理4d后，进行免疫细胞化学和兴奋性功能的检测，观察比较了不同培养方式和不同培养时间下EBs分化出来的神经细胞所占的比例。结果表明，用单纯悬浮3d或悬滴3d转悬浮1d的培养方法得到的胚体其神经分化的比例较高，这对进一步阐明胚胎干细胞自身内部调控诱导分化的机制有一定的参考意义。This study was directed at assessing the effects of embryonic bodies on differentiation of the embryonic stem cells. The embryonic bodies of different days gained by means of hanging, suspending and hungsuspended were processed by all-trans retinotic acids for 4 days. Then they were detected by immunocytochemistry methods and were measured to detect the fluorescence intensity changes of calcium after Glu stimulation. During this period, we observed the differentiation process and compared the differentiation ratio of embryonic stem cells processed by means of hanging, suspending for several days. It was found that the differentiation ratio of EBs obtained by suspending for 3 days, or by hanging for 3 days and suspending for 1 day, is relatively higher, which can help us find out the intrinsic differentiation mechanism of embryonic stem cells.

关 键 词：胚胎干细胞 神经分化 视黄酸诱导 胚体 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]