植物雌激素α-玉米赤霉醇对大鼠胸主动脉血管的舒张效应  

Relaxing effects of phytoestrogen alpha-zearanol on the thoracic aorta in rats

作　　者：王雯[1] 朱盈芬[1] 蒋东桥[1] 邱笑违[1] 王红霞[1] 曾翔俊[1] 张立克[1] 

机构地区：[1]首都医科大学病理生理学教研室,北京市100069

出　　处：《中国临床康复》2006年第23期87-89,共3页Chinese Journal of Clinical Rehabilitation

基　　金：北京市优秀人才培养专项经费(20042D0501817);首都医科大学自然科学科研基金(2004ZR02)~~

摘　　要：目的:观察植物雌激素α-玉米赤霉醇对大鼠胸主动脉的舒张作用及其途径。方法:实验于2005-09/12在首都医科大学病理生理学教研室完成。选取健康成年雌性Wistar大鼠17只,采用体外血管灌流的方法,用10-6mol/L苯肾上腺素预收缩血管,将10-6mol/L乙酰胆碱对血管环是否有舒张反应作为判断血管内皮是否完整的标准。观察α-玉米赤霉醇对血管内皮完整和去内皮胸主动脉的舒张作用,以及内皮型一氧化氮合酶抑制剂左旋硝精氨酸甲酯、鸟苷酸环化酶抑制剂亚甲蓝、钙激活钾通道阻断剂四乙胺、ATP敏感钾通道的阻断剂格列本脲、L-型钙通道激动剂(-)BayK8644(10-6mol/L)及雌激素受体阻断剂ICI182,780对这一过程的影响。各种药物对血管环的作用强度用最大舒张率表示,即加药后收缩幅度与加药前收缩幅度的百分比。结果:纳入大鼠17只,共取得胸主动脉环68个,共8个不合格予以剔除,其余60个胸主动脉环均用于实验。①α-玉米赤霉醇(10-10～10-5mol/L)可引起内皮完整的胸主动脉剂量依赖性的舒张效应;去内皮血管的最大舒张率显著低于同浓度内皮完整血管(P<0.05～0.01)。②左旋硝精氨酸甲酯和亚甲蓝均可抑制α-玉米赤霉醇对胸主动脉的舒张作用,使两组胸主动脉环的最大舒张率显著降低(P<0.05～0.01)。③四乙胺、格列本脲、BayK8644可以减弱α-玉米赤霉醇的舒张血管作用(P<0.05～0.01),雌激素受体特异性阻断剂ICI182,780对10-6mol/Lα-玉米赤霉醇的舒张作用无显著影响(P>0.05)。结论:α-玉米赤霉醇可引起内皮依赖性的胸主动脉舒张,其作用与一氧化氮合酶/一氧化氮系统激活、钙激活钾通道和ATP敏感钾通道开放、钙通道抑制有关,但与雌激素受体无关。α-玉米赤霉醇的舒张血管效应可能是其心血管保护途径之一。AIM： To observe the relaxing effects and mechanism of phytoestrogen α-zearanol on thoracic aortas in rats. METHODS： The experiment was conducted in the Department of Pathophysiology, Capital University of Medical Sciences between September and December 2005. A total of 17 healthy male adult Wistar rats were selected. Method of in vitro vascular perfusion was adopted to pre-contract the vessels with 104 mol/L of phenylephrine. The relaxing effects of aeetylcholine （104 moL/L） on vascular ring was taken as the judging standard of that whether the blood vessel endothelium was intact. The relaxing effects of α-zearanol on the blood vessel endothelium and thoracic aortas without endothelium were observed, and the influence of Nω-nitro-L-arginine methylester （L-NAME, nitric oxide synthase inhibitor）, methylene blue （guanylate cyclase inhibitor）, tetraethylammonium （calcium-activated potassium channel blocker）, giibenclamide （ATP-sensitive potassium channel blocker）, （-）BayK8644 （L-type calcium channel agonist） and ICI182,780 （estrogen receptor antagonist ） on tho procedure were observed. The intensity of effects of all drugs on vascular ring was presented by the maximum diastolic rate,i.e. the percentage of contracted extent after and before administration. RESULTS： A total of 68 thoracic aortas rings were obtained from 17 enrolled rats, 8 of which were excluded for disqualification, and the rest 60 rings were used in the experiment.①α-zearanol （10^-10-10^-5 mol/L） could induce an concentration-dependent relaxation in intact thoracic aortas, while the maximum diastolic rate of vessels without endothelium was significantly lower than vessels with intact endotbelium （P 〈 0.05-0.01）.②L-NAME could inhibit the relaxing effects of α-zearanol on thoracic aortas, which could obviously decrease the maximum relaxing rate of thoracic aortas rings in beth groups （P 〈 0.05-0.01）. ③Tetraethylammonium, giibenclamide and BayK8644 could also reduce the relaxing effect

关 键 词：植物雌激素类 主动脉 胸 内皮 血管 一氧化氮 钾通道 钙通道 

分 类 号：R286.2[医药卫生—中药学]