γ射线诱发的白血病小鼠骨髓细胞SHP-1 mRNA及蛋白表达变化的研究  

Changes of SHP-1 mRNA and protein of bone marrow cells of mice with leukemia induced by γ-ray irradiation

作　　者：黄定德[1] 陈杞[2] 韩玲[2] 蔡建明[2] 

机构地区：[1]第三军医大学西南医院核医学科,重庆400038 [2]第二军医大学海军医学系放射医学教研室,上海200433

出　　处：《第三军医大学学报》2006年第12期1295-1297,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30170282)~~

摘　　要：目的探讨蛋白酪氨酸磷酸酶SHP-1 mRNA及蛋白表达在γ射线体外诱发的白血病小鼠骨髓细胞中的变化情况。方法实验分为白血病组、辐射未癌变组及对照组,应用荧光定量PCR法及W estern b lot法分别检测各组骨髓有核细胞中SHP-1 mRNA及蛋白表达的变化情况。结果白血病组SHP-1 mRNA含量为(5.26±2.14),明显高于辐射未癌变组(3.68±1.27)及对照组(2.95±1.09),(P<0.01);白血病组SHP-1蛋白表达量(光密度)为(5.378±2.905),明显高于对照组(1.852±0.711)及辐射未癌变组(1.559±0.506)。结论在γ射线诱发的白血病小鼠骨髓细胞中,SHP-1 mRNA含量及蛋白表达量均明显增强。Objective To investigate alteration of SHP-1 mRNA and protein of bone marrow cells of leukemia mice after γ-ray exposure. Methods A total of 318 BALB/c mice were exposed to ^60Co γ-ray once a week for 4 weeks, and the total dose of γ-ray received by mice was 7.00 Gy. By pathological examination, 39 mice developed thymic lymphoma, 14 acute lymphocytic leukemia, 21 T-lymphoblast leukemia/lymphoma, 1 spiroma, 4 malignant yolk sac tumor. Exposed to γ-ray, the mice that developed leukemia or were free of canceration were used in our study （n = 10 in each group） , and another 10 mice free from irradiation served as control. SHP-1 mRNA and protein in femoral bone marrow cells were detected by real-time fluorescence quantitative PCR （FQ-PCR） and Western blotting respectively. Results SHP-1 mRNA in leukemia mice was （5.26±2.14）, significantly higher than that of mice free of canceration （3.68 ± 1.27） and controls （2.95 ± 1.09） （ P 〈 0.01 ）. SHP-1 protein in leukemia mice [ optical density （5. 378 ± 2. 905 ） ] was significantly higher than that in mice free of canceration [ optical density （ 1. 559 ± 0.506 ） ] and controls [ optical density （ 1. 852 ± 0.711 ） ]. Conclusion SHP-1 mRNA and protein were significantly increased in bone marrow cells of leukemia mice induced by γ-ray irradiation.

关 键 词：辐射致癌 信号转导 SHP-1 小鼠 白血病 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R331.22[医药卫生—基础医学]