LMO3 mRNA在小鼠中枢神经系统的表达定位  

Localization of neural specific transcription factor LMO3 mRNA in adult mouse central nervous system

作　　者：惠玲[1] 蔡文琴[1] 吕同德[2] 

机构地区：[1]第三军医大学基础医学部神经生物学教研室重庆市神经科学研究所,重庆400038 [2]兰州军区兰州总医院医学科学实验中心,兰州730050

出　　处：《第三军医大学学报》2006年第13期1367-1369,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目青年基金资助项目(30400125)~~

摘　　要：目的 观察神经特异性转录因子LMO3mRNA在小鼠中枢神经系统中的定位。方法 原位杂交组织化学染色。结果LMO3mRNA阳性神经元可见于大脑、小脑、丘脑、脑干、脊髓，在小鼠中枢神经系统中，LMO3mRNA阳性反应产物主要位于细胞质和突起内。强阳性信号主要出现于大脑皮质的Ⅱ一Ⅵ层、梨状皮质、内嗅皮质、海马的CA1区、齿状回、中脑的黑质致密部、红核、室旁核、巨细胞网状核、外侧网状核、三叉神经脑桥核、面神经核、三叉神经运动核、舌下神经核等部位；中等强度着色主要分布于扣带前皮质、嗅球、海马的CA2区、梨状内核、杏仁核等部位；海马的CA3区、尾壳核、苍白球、杏仁复合体、丘脑、三叉神经脊束核、疑核、中缝核团、小脑间置核及外侧核、浦肯野细胞可见弱的阳性细胞。结论LMO3基因在CNS的广泛分布提示它除了在多巴胺神经递质的活动中起作用外，还参与了机体的学习记忆、嗅觉的调节。Objective To explore the localization of neural specific transcription factor LMO3 mRNA in the central nervous system of the mice. Methods Five male adult mice, weighing 60 - 80 g, were sacrificed, and the brain and spinal cord were fixed and sliced. In situ hybridization histochemistry staining method with digoxigen-lablled cRNA probe was used in detection of LMO3 mRNA. Results The expression of LMO3 mRNA could be observed in cerebrum, cerebellum, thalamus, brain stem and spine. They were localized in somatic and dendritic profiles at most regions of adult mouse central nervous system. The intense hybridization signal could be seen in Ⅱ-Ⅵ layers of cerebrum, piriform cortex, entorhinal cortex, hippocampus CA1 and dentate gyrus, substantia nigra, motor nucleus of trigeminus, nucleus of hypoglossal, red nucleus, paraventricular nucleus, gigantocellular reticular nucleus, lateral reticular nucleus. The moderate staining was detected in olfactory bulb, hippocampal CA2, amygdala nucleus, cingulated anterior cortex, piriform nucleus etc.. The faint signal showed in globus pallidus, spinal nucleus of trigeminal, interposed cerebellar nucleus, ambiguous nucleus, lateral cerebellar nucleus, Purkinje cell of cerebellum, thalamus, caudate putamen, amygdaloid body, nuclei raphe. Conclusion The wide presence of LMO3 mRNA in adult mice central nervous system suggests a role of regulation for this gene in learning, memory and olfaction besides in dopamine neurotransmitter regulation.

关 键 词：LMO3 LIM-only 中枢神经系统 原位杂交组织化学 小鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R394-33[医药卫生—基础医学]