新型异黄酮及甾体类化合物体外抗癌细胞增殖活性的初步筛选  

Screening cell proliferation activity of novel isoflavone and steroid derivatives

作　　者：张丽龙[1] 粟永萍[1] 刘晓宏[1] 程天民[1] 郑虎[2] 翁玲玲[2] 艾国平[1] 

机构地区：[1]第三军医大学预防医学院防原医学教研室全军复合伤研究所创伤烧伤与复合伤国家重点实验室,重庆400038 [2]四川大学华西药学院,成都610041

出　　处：《第三军医大学学报》2006年第13期1393-1396,共4页Journal of Third Military Medical University

基　　金：重庆市院士基金资助项目(2004BC5006)~~

摘　　要：目的研究改构后的异黄酮及甾体类化合物是否具较好的体外抗细胞增殖的活性。方法采用MTT法,以5,7,4’-三羟基异黄酮(Gen iste in,GEN)作为对照,研究28个改构后的异黄酮和甾体化合物对Hela细胞、MCF-7细胞的抗增殖作用,并在此基础上,分析其构效关系。结果通过对28种化合物采用MTT的方法对其体外活性进行初筛,初步认为异黄酮类化合物F11、ZF3、ZF4、ZF7以及甾体类化合物ZE2均对Hela细胞、MCF-7细胞增殖有显著的抑制作用,效果均优于Gen iste in。结论异黄酮和甾体类化合物经过结构改造和修饰,其大部分对Hela细胞、MCF-7细胞的增殖有显著的抑制作用,有进一步研究的潜在价值。Objective To investigate whether novel isoflavone and steroid derivatives have better antitumor activity. Methods The anti-proliferation activities of 28 novel isoflavone and steroid derivatives upon Hela cells and MCF-7 cells were analyzed by MTT, then compared with that of Genistein. Results Raw screening of the 28 compounds by MTT indicated isoflavone derivatives F11, ZF3, ZF4, ZF7 and steroid derivatives ZE2 exerted more obvious suppression on the proliferation of tumor cell lines than Genistein. Conclusion Most of the novel isoflavone and steroid derivatives inhibit the proliferation of tumor cell lines. The com- pounds obtained from the screening have more potent cytotoxicity on tumor cells than Genistein and are worth further investigation.

关 键 词：异黄酮 甾体 MTT 

分 类 号：R285.5[医药卫生—中药学] R329.28[医药卫生—中医学]