丙型肝炎病毒慢性感染与IL-8、IP-10、Mig表达相关性研究  

Relationship between HCV chronic infection and expression of IL-8,IP-10,Mig

作　　者：王健[1] 赵金红[1] 江水清[2] 项桂菊[2] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室,淮南232001 [2]淮南第二矿工医院感染科,淮南232052

出　　处：《第三军医大学学报》2006年第13期1420-1423,共4页Journal of Third Military Medical University

基　　金：安徽省教育厅自然科学基金资助重点项目(2003kj027zd)~~

摘　　要：目的探讨HCV慢性感染与CXC趋化因子mRNA表达水平的相互关系。方法以Real-tim e PCR法动态检测慢性丙肝患者IFN-α联合利巴韦林治疗前、治疗3、6个月后IL-8、IP-10、M ig mRNA的表达水平。以趋化因子与GAP-DH比值为趋化因子的最终相对表达量。结果慢性丙肝患者HCV-RNA平均水平2.09×106拷贝/m l,IL-8、IP-10、M igmRNA表达水平分别为(1.243 7±0.071 2)、(0.682 8±0.073 8)、(0.668 3±0.075 4),与正常对照相比,差异有显著性(P<0.01)。IFN-α治疗3个月后HCV-RNA平均水平6.70×103拷贝/m l,IL-8、IP-10、M ig mRNA表达水平分别为(1.089 1±0.094 6)、(0.551 2±0.090 2)、(0.560 2±0.096 0),与治疗前相比,差异有显著性(P<0.001)。IFN-α治疗6个月后HCV-RNA平均水平3.86×102拷贝/m l,IL-8、IP-10、M ig mRNA表达水平分别为(1.012 2±0.058 6)、(0.439 5±0.091 3)、(0.420 5±0.084 4),与治疗前相比,差异有显著性(P<0.01)。HCV 1b和HCV 2 a型患者各趋化因子表达水平相似,差异无显著性(P>0.05)。结论慢性丙肝患者PBMC内IL-8、IP-10、M ig mRNA表达升高,其表达水平与体内HCV-RNA复制水平有关,但与HCV基因型无关。IFN-α能显著抑制HCV复制,间接下调IL-8、IP-10、M ig mRNA表达水平。IFN-α这种综合作用效果,对降低患者体内炎性反应,控制病情发展十分有利。Objective To study the relationship between HCV chronic infection and expression of CXC chemokines mRNA. Methods The levels of IL-8, IP-10 and Mig mRNA in peripheral blood mononuclear cells （PBMC） of chronic hepatitis C patients were detected by real-time quantitative polymerase chain reaction （RT-PCR） before, and 3, 6 months after combined therapy of interferon-α （IFN-α） and Ribavirin. The rate of chemokine/GAPDH was regarded as the extreme level of chemokine. Results The mean level of HCV-RNA and expressive levels of IL-8, IP-10, Mig mRNA in peripheral blood of HCV chronic infection were 2. 09 × 106 copies/ml, and （1. 243 7 ±0. 071 2）, （0. 682 8 ±0. 073 8）, （0. 668 3 ±　0. 075 4）, with significant difference with those of normal controls （ P 〈0.01 ）. After 3-month treatment, the mean level of HCV-RNA and the expressive levels of IL-8, IP-10, Mig mRNA were 6. 70× 103copies/ml, （ 1. 089 1 ±0. 094 6） , （0. 551 2 ±0. 090 2）, （0. 560 2 ± 0. 096 0）, respectively. After 6-month treatment, the mean level of HCV-RNA and the expressive levels of IL-8, IP-10, Mig mRNA were 3.86 × 102copies/ml, （ 1. 012 2 ±0. 058 6）, （0. 439 5 ± 0. 091 3）, （0. 420 5 ±0. 084 4）, respectively. There was a significant difference before and after treatment （P 〈0. 01 ）. Similar expression of chemokines was found in the patients with gene type of HCV lb and HCV 2a （P 〉 0. 05）. Conclusion The expressions of IL-8, IP-10 and Mig mRNA in PBMC are high in patients with chronic hepatitis C, and associated with the replication level of HCV, and less associated with the gene type of HCV. IFN-α can effectively restrain the replication of HCV and down-regulate the levels of IL-8, IP-10 and Mig mRNA near to normal level in patients with chronic hepatitis C. IFN-ct plays an important role in down-regulating inflammation response and controlling the development of the patients＇ condition.

关 键 词：丙型肝炎 趋化因子 mRNA real-time PCR 

分 类 号：R392.13[医药卫生—免疫学] R512.63[医药卫生—基础医学]