钙荧光探剂的研究及其在生命科学中的应用  

Development of Calcium Fluorescent Probes and Their Application in Life Sciences

作　　者：张川里[1] 吴本[1] 

机构地区：[1]北京医科大学生物物理学系

出　　处：《生理科学进展》1996年第1期37-42,共6页Progress in Physiological Sciences

摘　　要：钙荧光探剂测量活细胞胞浆游离Ｃａ２＋浓度的方法在钙研究中已成为一种越来越重要的技术。特别是由于新的一代荧光探剂的合成和激光共聚焦显微镜的发展，使其应用更加广泛。由于国内使用这种技术的实验室逐渐增多，本文将系统介绍钙荧光探剂的发展、测量原理和方法、新的常用钙荧光探剂的比较及其在生命科学中的应用。t is well known that calcium is the key second intracellular messenger.Techniques using calcium fluorescent probes have been rapidly developed during the last decade,and have become one of the most important methods to measure intracellular free ca2+concentrations. This trend was even more accelerated after the development of a new generation of calcium fluorescent probes and the laser scanning confocal microscopy In this article we will review the development of calcium fluorescent probes,the mechanisms and methods of measuring intracellular free ca2+ concentrations,the properties of new calcium fluorescent probes, and their applications in life sciences.

关 键 词：细胞 钙荧光探剂 钙离子浓度 细胞学技术 

分 类 号：Q2－33[生物学—细胞生物学]