抗D－双聚体单抗轻链可变区基因的克隆  

Molecular Cloning of Kappa Variable Domain Against Human D-Dimer

作　　者：官孝群[1] 张艳玲[1] 李勇 丁皓[1] 朱运松[1] 宋后燕[1] 

机构地区：[1]上海医科大学基础医学院

出　　处：《生物化学与生物物理进展》1996年第1期65-69,共5页Progress In Biochemistry and Biophysics

基　　金：国家"863"计划资助

摘　　要：以分泌小鼠抗人纤维蛋白降解产物Ｄ－双聚体单克隆抗体杂交瘤细胞株的ｍＲＮＡ为模板，用小鼠免疫球蛋白轻链可变区基因的通用引物，通过ＲＴ－ＰＣＲ扩增基因，与载体重组后，经酶切鉴定和核苷酸序列分析，证明克隆含抗Ｄ－双聚体单抗的轻链可变区基因，长度为３３０ｂｐ．Using the mRNA prepared from hybridoma DW10 secreted monoclonal antibody against human D-dimer as a template, thecDNA fragment encoding the Vk domain was amplified by reverse transcription and polymerase chain reaction (RT-PCR) with a set of universe primers that were on the FR1 region and FR4 region of Vk domain respectively.The RT-PCR product was ligated with pUC18. The result obtained from the restriction enzyme fragments and DNA sequencing showed that the length of the Vk was 330 bp,and the distinctive structures of the immunoglobin CDR1, CDR2 and CDR3 of Vkgene appeared.

关 键 词：抗D-双聚体 单克隆抗体 轻链可变区 基因克隆 

分 类 号：R392－33[医药卫生—免疫学] R394.8[医药卫生—基础医学]