钙调素一级结构保守性和变异性的数量分析Ⅱ钙结合区和疏水穴保守性和变异性的数量分析  

QUANTITATIVE ANALYSIS OF CONSERVATISM AND VARIABILITY OF CALMODULIN PRIMARY STRUCTURE Ⅱ QUANTITATIVE ANALYSIS OF CONSERVATISM AND VARIABILITY OF Ca￣(2+) -BINDING LOOP AND HYDROPHOBIC CLEFT

作　　者：马力耕[1] 陆运青[1] 孙大业[1] 阎隆飞[1] 

机构地区：[1]河北师范大学生物系,北京农业大学植物生理生化开放实验室,河北师范大学教育系

出　　处：《生物物理学报》1996年第1期1-7,共7页Acta Biophysica Sinica

基　　金：北京农业大学植物生理生化开放实验室资助

摘　　要：用计算机为工具对３２种生物钙调素（ＣａＭ）的四个钙结合区和两个疏水穴一级结构的保守性和变异性进行了数量分析。结果表明第一、二钙结合区（Ｎ端）比第三、四钙结合区（Ｃ端）更保守；即大多数物种ＣａＭ的第一、二钙结合区的同源性大于它们全序列间的同源性。而第三、四钙结合区则因亲缘关系和高低等生物而异，情况较复杂。Ｎ端疏水穴比Ｃ端疏水火保守，前者的同源性高于它们全序列间的同源性；而后者亦因亲缘关系及高低等生物而异规律不明显。The conservatism and variability of calmodtilin (CaM) primary structures of 32 organisms were analyst quantitatively by the aid of microcomputer. The results showed that the first and second Ca2+-binding loops (located at N-terminal) were more conservative than the third and fourth loops (Localed at C-terminal) and the similarities of the first and second Ca2+-binding loops were greater than those of complete sequences for most of the organisms.The N-terminal hydrophobic cleft was more conservative than that at C-terminal, and the similarites of the former were greater than those of complete sequences. The changing patterns of similarites of the third, fort Ca2+-binding loops and the C-terminal hydrophobic clefts were more complicated, which might depend on the evolutionary relationships and levels of dinferent orgamisms.

关 键 词：调钙蛋白 一级结构 保守性 变异性 数量分析 

分 类 号：Q510.1[生物学—生物化学] Q617