共轭亚油酸对胃癌MGC-803细胞增殖的影响  

Effect of Growth Inhibition of Conjugated Linoleic Acid on Gastric Cancer MGC - 803 Cell Line in Vitro

作　　者：贺栋梁[1] 李小玲[1] 李东阳[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《美国中华临床医学杂志》2006年第3期282-284,共3页American Journal of Chinese Clinical Medicine

摘　　要：目的 研究共轭亚油酸（CLA）对体外培养人胃癌MGC-803细胞生长的影响。方法 采用体外培养人胃癌MGC-803细胞，用MTT比色、流式细胞光度术以及Cyclin D1蛋白表达检测的方法，观察不同浓度CLA（50．0、100．0、200．0μmol／L）对MGC-803细胞生长的影响。结果 MTT比色结果显示，不同浓度CLA可抑制人胃癌MGC-803细胞的增殖，72h的抑制率分别为48．61％、62．02％、66．33％，呈现剂量-效应关系（P〈0．05）。流式细胞仪检测表明，不同浓度CLA（50．0、100．0、200．0μmol／L）作用MGC-803细胞48h，C1期细胞从0μmol／L浓度组的32．2％分别上升到43．1％、59．7％、63．9％，出现G1期阻滞。免疫组织化学结果显示，Cyclin D1蛋白表达随着CLA浓度的增加呈现逐渐下降的趋势。结论 CLA对体外培养的人胃癌MGC-803细胞增殖有良好的抑制作用。Objective To study the effects of different concentration of conjugated linoleic acid on gastric cancer MGC - 803 cell line growth in vitro. Methods The influence of different concentrations of CLA on gastric cancer MGC - 803 cell llne was examined by MTT, flow cytometry （FCM） and immunocytochemical staining assay. Results Suppression and decrease of MGC - 803 cell proliferation was found after treatment by CLA in vitro. The inhibitory rates on MGC - 803 cell growth of different concentration of CLA （50.0,100.0,200.0 μmol/L） were 48.61%, 62.02%, 66.33%. There was significant correlation between dose and effect. Immunocytochemical staining and FCM indicated that CLA down - regulated the expression of Cyclin DI protein and arrest cell cycle in G1 phase. Conclusion The effect of growth inhibition of CLA on MGC - 803 cell in vitro is remarkable.

关 键 词：MGC-803细胞 CLA 增殖 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]