高原适应过程中大鼠脑内β－内啡肽含量变化的研究  

EXPERIMENTAL STUDY OF THE CONTENTS OF β-ENDORPHIN IN RAT BRAIN DURING ADAPTATION TO HIGH ALTITUDE

作　　者：谢新民[1] 孟宪纪[1] 白若华[1] 

机构地区：[1]青海医学院生理学教研室

出　　处：《中国应用生理学杂志》1996年第1期33-36,共4页Chinese Journal of Applied Physiology

基　　金：青海省卫生厅资助

摘　　要：选用成年雄性Ｗｉｓｔａｒ大鼠，由平原（海拔５ｍ，上海）直接运送到高原（２２６１ｍ，西宁和３４６０ｍ，天峻）。以放射免疫分折法研究了大鼠在高原２４ｈ．急性缺氧期和３０ｄ慢性缺氧期中枢脑内β－内啡肽样免疫活性物质（β－ＥＰ）的含量变化。结果表明：１．大鼠在两个不同海拔（２２６１ｍ，３４６０ｍ）环境２４ｈ急性缺氧期与平原对照组相比，脑垂体内β－ＥＰ含量降低非常显著（Ｐ＜０．０１），纹状体、丘脑、下丘脑、桥－延脑、海马内β－ＥＰ含量均增加非常显著（Ｐ＜０．０１）。２．大鼠在３４６０ｍ高原３０ｄ慢性习服期，垂体及各脑区内β－ＥＰ的含量变化呈时相性：即１—３ｄ均呈进行性增加（Ｐ＜０．０１）：３－１５ｄ呈持续性减少，除中脑、纹状体、丘脑外，均为Ｐ＜０．０１。１５－３０ｄ垂体、丘脑、皮层、纹状体内β－ＥＰ含量仍持续减少（毒体、皮层Ｐ＜０．０１），桥－延脑、下丘脑、海马趋于回升（Ｐ＜０．０１），中脑亦趋于回升（Ｐ＞０．０５）。脑内β－ＥＰ的这种变化可能具有十分重要的生物学意义。The contents of βvendorphin(β-EP)the brain of rats which were taken from plain area(5m above sea level)to plateau area(22261 m and 3 460m above sea level)in acute hypoxia(24hour)and chronic adaptation period(30 day)were studied by means of the radioimmunoassay(RIA).The resuIts showed that the content ofβ-EP in hypophysis was decreased(P<0.01).However,it was increased in corpus striatum,thalamus,hypothalamus,pons-oblongata andhippocampus(P<0.01)in acute reaction period(24h).Moreover,the contents of β-EP in all 8brain areas were increased(P<0.01) in acute adaptation period(1-3d),those in all the brainareas were decreased(P<0.01)during 3-15d and in part of these areas decreased continually(P<0.01)during 15-30d.The above results indicate that the β-EP in the brain may take part inthe regulation of adaptation at high altitude.

关 键 词：高原适应 Β-内啡肽 含量变化 

分 类 号：R339.54[医药卫生—人体生理学]