高效液相色谱法测定大蒜中吡虫啉残留  

Determination of Imidacloprid Residue in Garlic by HPLC

作　　者：王勇[1] 周宏琛[1] 闫秋成 朱涛[1] 田晓林[1] 

机构地区：[1]济宁出入境检验检疫局,山东济宁272025

出　　处：《济宁师范专科学校学报》2006年第3期16-17,共2页Journal of Jining Teachers College

摘　　要：建立了大蒜中吡虫啉残留分析的高效液相-二极管阵列检测方法.样品经甲醇提取浓缩后,用5%氯化钠水溶液及石油醚萃取,再用二氯甲烷反萃取水相,吹干,定容,采用反向C18色谱柱,流动相为甲醇/水（体积比32： 68）,波长为272m.结果表明：在0.1～10.0μg/mL范围内回归方程为：Y=126.208X-1.348,相关系数为0.99998,呈良好的线性关系,方法检出限为0.05mg/kg,平均回收率为79.6%～88.2%,相对标准偏差为2.1%～3.2%.A method for the determination of Imidacloprid in garlic was established by HPLC with DAD detector. Residues of Imidacloprid were extracted from sample with methanol, liquid-liquid extraction cleanup with petroleum ether and then with dichloromethane to extract the samples further. The HPLC conditions were RP--C18 column, methanol-water 32：68 （V/V） as mobile phase,and wavelength at 272nm. The linear range was 0. 1-10. 0μg/mL（r= 0. 99998）and the regression equation was Y= 126. 208X-1.348. The detection limit of Imidacloprid was 0. 05mg/kg in the sample of garlic and the ranges of average recoveries were 79.6-88.2%, RSD was 2.1-3.2%.

关 键 词：高效液相色谱法 吡虫啉 大蒜 

分 类 号：Q93-332[生物学—微生物学]