+Gz重复暴露后大鼠TNF-α和IL-1β水平及肺组织中mRNA表达变化  

Changes of content and mRNA expression level of TNF-α and IL-1β in rats’ lungs after repeated exposure to +Gz

作　　者：李燕玉[1] 徐丽梅[2] 张波[2] 刘又宁[1] 

机构地区：[1]解放军总医院呼吸内科,北京100853 [2]空军总医院呼吸内科,北京100036

出　　处：《军医进修学院学报》2006年第3期218-219,共2页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的:研究不同水平正加速度(+Gz)重复暴露后,大鼠血清和肺泡灌洗液(BALF)中TNF-α和IL-1β含量及其在肺组织的mRNA表达水平的变化,探讨这两种致炎因子在+Gz重复暴露肺损伤中的可能作用。方法:雄性Wistar大鼠20只,随机分为对照组(+1Gz)、+5Gz组、+10Gz组和+15Gz组。采用梯形加速度曲线,在动物离心机上离心,+1Gz、+5Gz和+10Gz各暴露90s,+15Gz暴露30s,均间隔30min重复3次。于暴露后4h取大鼠腹腔静脉血、BALF及肺组织,用放免法测定血清和BALF中IL-1β和TNF-α含量;用RT-PCR方法检测大鼠肺组织中TNF-α和IL-1βmRNA表达水平。结果:+Gz暴露后大鼠血清和BALF中TNF-α和IL-1β含量及其在肺组织中mRNA表达水平明显高于对照组,并随着+Gz强度的升高而升高。结论:炎症介质TNF-α和IL-1β在+Gz暴露导致大鼠肺组织的生物性损伤中可能起着重要的作用。Objective： To study the changes of content of TNF-α and IL-1β in rat serum and BALF and the mRNA expression level in rat lungs after repeated exposure to ＋Gz, so as to investigate the mechanism of lung injure induced by acceleration. Methods： 20 male health Wistar rats were randomly divided into 4 groups （n =5） ： normal control group（ ＋ 1Gz）, ＋ 5Gz group, ＋ 10Gz group and ＋ 15Gz group. Using animal＇s centrifuge, the profile of the centrifuge ＋ Gz exposure was trapezoidal, in which ＋ 15Gz group lasted for 30s and the other three groups lasted for 90s respectively. All single profiles were repeated 3 times with 30min interval. The blood sample, BALF and lung tissues were taken 4h after the last centrifuge run. The contents of TNF-α and IL-1β in the serum and BALF were measured by radioimmunoassay and the mRNA expression levels in rat lungs were measured by semi-quantitative reverse transcription poly-merase chain reaction （RT-PCR）. Results： The contents of TNF-α and IL-1β in rat serum and BALF and the mRNA expression levels in the rat lungs 4h later after repeated exposure to ＋ Gz were significantly higher than those in controls, and increased parallel with the ＋ Gz. Conclusion：It demonstrated the inflammation mediators TNF-α and IL-1β maybe play an important role in the biological damage of rat lung induced by repeated ＋ Gz exposure.

关 键 词：加速度 大鼠 炎症介导素类 基因表达 

分 类 号：R563[医药卫生—呼吸系统]